复方七芍降压片对自发性高血压大鼠肠系膜动脉中Ⅰ、Ⅲ型胶原蛋白表达及血压的影响  

Compound Qishao Jiangya tablet reduces blood pressure in mesenteric artery of SHR rats by decreasing the deposition of type Ⅰ and type Ⅲ collagen

作　　者：李弘 刘叶倩 龚姗 刘林[1,2] 曾勇[1,2] 蔡晓[1,2] 王宇红[1,2] 任卫琼[1,2] LI Hong;LIU Yeqian;GONG Shan;LIU Lin;ZENG Yong;CAI Xiao;WANG Yuhong;REN Weiqiong(The First Hospital of Hunan University of Chinese Medicine,Changsha 410007,Hunan,China;Innovation Centre for Science and Technology,Changsha 410208,Hunan,China)

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]湖南中医药大学科技创新中心,湖南长沙410208

出　　处：《中国临床药理学与治疗学》2019年第12期1364-1370,共7页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：湖南省中医药管理局重点项目(201910);湖南省科技厅项目(2018SK51203);中医内科重大疾病防治及转化教育部重点实验室开放基金(ZYNK201703);研究生校级创新课题(2018CX37);国家自然科学基金(81473616)

摘　　要：目的:探究复方七芍降压片对自发性高血压大鼠(SHR)血压及血管重塑的作用机制。方法:SHR大鼠随机分为模型组、厄贝沙坦片组、复方七芍降压片组,每组10只,另设10只WKY大鼠为正常对照组,给药6周,无创血压仪测量各周大鼠血压变化;HE染色观察大鼠肠系膜动脉病理变化并测量管壁厚度、血管中膜厚度及相同位置的管腔直径,比值记为WT;免疫组化法检测肠系膜动脉中基质金属蛋白酶-2(MMP-2)及Ⅰ、Ⅲ型胶原蛋白(COⅠ、COⅢ)表达情况。结果:给药6周后,与正常对照组比较,模型组大鼠各周血压均明显升高(P<0.01),管壁厚度及WT值增高(P<0.01或P<0.05),肠系膜动脉中MMP-2及Ⅰ、Ⅲ型胶原蛋白表达均明显升高(P<0.01);与模型组比较,复方七芍降压片组大鼠各周血压均明显降低(P<0.01),管壁厚度及WT值降低(P<0.05),肠系膜动脉中MMP-2及Ⅰ、Ⅲ型胶原蛋白表达均明显降低(P<0.05),且病理变化得到改善。结论:复方七芍降压片具有降低SHR大鼠血压,改善血管重塑的作用,其机制可能与降低MMP-2表达及Ⅰ、Ⅲ型胶原蛋白沉积有关。AIM:To explore the mechanism of compound Qishao Jiangya tablet on blood pressure and vascular remodeling in spontaneously hypertensive rats(SHR).METHODS:SHR rats were randomly divided into model group,irbesartan tablet group and compound Qishao Jiangya tablet group,10 rats in each group;Another 10 WKY rats were used as normal control group;All received administration for 6 weeks;noninvasive sphygmomanometer was used to measure the changes of blood pressure in rats;HE staining was used to observe the pathological changes of mesenteric artery in rats and to measure the thickness of mesenteric artery wall;The thickness of intima media and the diameter of lumen in the same position were recorded as WT;Detection of matrix metalloprotease-2(MMP-2)and typeⅠand type Ⅲ collagen(COⅠ,CO Ⅲ)protein expression in mesenteric artery was performed by immuno-histochemical method.RESULTS:After 6 weeks of administration,compared with the normal control group,the blood pressure of the model group was significantly higher than that of the control group(P<0.01),and the thickness of tube wall and WT value were increased(P<0.01 or P<0.05),the expression of MMP-2,I and Ⅲ collagen in mesenteric artery increased significantly(P<0.01);Compared with model group,the blood pressure of the rats in the compound Qishao Jiangya tablet group was significantly lower than that in the control group(P<0.01),The thickness of tube wall and WT value decreased(P<0.05),the expression of MMP-2 and typeⅠand type Ⅲ collagen in mesenteric artery was significantly decreased(P<0.05),and the pathological changes were improved.CONCLUSION:Compound Qishaojiang tablet can reduce blood pressure and improve vascular remodeling in SHR rats,the mechanism may be related to the decrease of MMP-2 expression and the deposition of typeⅠand type Ⅲ collagen.

关 键 词：复方七芍降压片 肠系膜动脉 基质金属蛋白酶-2 I型胶原蛋白 Ⅲ型胶原蛋白 血管重塑 

分 类 号：R965.2[医药卫生—药理学] R544[医药卫生—药学]