大黄花虾脊兰种子特性及无菌播种  

Seed characteristics and aseptic seeding of Calanthe striata

作　　者：刘海平 陈秀萍 李珺 吴沙沙[1,2] 何碧珠[2] 兰思仁[1,2] 翟俊文[1,2] LIU Haiping;CHEN Xiuping;LI Jun;WU Shasha;HE Bizhu;LAN Siren;ZHAI Junwen(College of Landscape Architecture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Guangzhou Institute of Forestry and Landscape Architeture,Guangzhou,Guangdong 510405,China)

机构地区：[1]福建农林大学园林学院,福建福州350002 [2]福建农林大学兰科植物保护与利用国家林业和草原局重点实验室,福建福州350002 [3]广东市林业和园林科学研究院,广东广州510405

出　　处：《福建农林大学学报（自然科学版）》2020年第1期29-34,共6页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金青年基金项目(31400180);国家林业局森林公园工程技术研究中心科学技术研究项目(PAH1500213)

摘　　要：以大黄花虾脊兰自然结实120~150d成熟未开裂的蒴果作为研究材料,对56个果荚中种子的有胚率进行统计,并采用TTC法测定种子活力,选取最佳用于无菌播种的果荚.以活力最佳的果荚为研究材料,对在不同含量的NAA、6 BA、KT及不同有机添加物下种子萌发及幼苗生长情况进行观察.结果表明:大黄花虾脊兰种子的有胚率为15.57%~95.66%,平均值为45.15%±2.68%,授粉后140 d的蒴果种子活性最佳;种子萌发120和140 d后种胚突破种皮生长为原球茎,150 d顶端分生出明显的芽生长点,底部生出肉质的不定根;较佳的培养基组合为:2 g·L^-1 Hyponex 1+1/2 MS+0.37 mg·L^-1 MgSO 47H2O+25 g·L^-1蔗糖+1.0 mg·L^-16 BA+0.2 mg·L^-1 NAA+1.0 mg·L^-1 KT+5.5 g·L^-1琼脂粉+1.5 g·L^-1活性炭+2 g·L^-1牛肉粉蛋白胨+50g·L^-1洋葱,pH5.7~5.8,培养(140±10)d可形成原球茎,萌发率达39.64%±3.34%.To develop a scale cultivation model for Calanthe striata R.Br.,120-150 days old mature and uncracked C.striata capsule obtained by natural seed setting were selected as the test material.Embryogenic rate of seeds from 56 pods was counted,and seed vigor was determined by TTC method to select the most vigorous seed for aseptic seeding.Then seed germination and seedling growth tests were conducted upon the pod with the best vitality under different concentrations of NAA,6BA,KT and organic additives using L9 orthogonal design.The results showed that the percentage of embryo in C.striata seeds ranged between 15.57%and 95.66%,averaging at 45.15%±2.68%.Capsule on the 140th day after seed set had the highest vitality.After 120 or 140 d,the embryo broke through the seed coat and grew into protocorm.After 150 d,obvious bud growing points were developed at the apical tissue,and fleshy adventitious roots were produced at the bottom.A preferred medium is the combination of 2 g·L^-1 Hyponex 1,1/2 MS,0.37 mg·L^-1 MgSO4·7H2O,25 g·L^-1 sucrose,1.0 mg·L^-16BA,0.2 mg·L^-1 NAA,1.0 mg·L^-1 KT,5.5 g·L^-1 agar powder,1.5 g·L^-1 activated carbon,2 g·L^-1 beef powder peptone,50 g·L^-1 onion under 5.7-5.8 pH.Protocorm was developed(140±10)d after cultivation,with the germination rate being 39.64%±3.34%.

关 键 词：大黄花虾脊兰 无菌萌发 种子活性 有胚率 

分 类 号：S682.31[农业科学—观赏园艺]