长期社会击败对小家鼠行为的影响及其神经内分泌机制  

The effect of chronic social defeat on the behavior of mice and the underlying neuroendocrine mechanisms

作　　者：景晓源 朱云萌 王艳 刘秀秀 邢梦迪 夏敏[1] 李来福[1] 刘莹娟[1] JING Xiaoyuan;ZHU Yunmeng;WANG Yan;LIU Xiuxiu;XING Mengdi;XIA Min;LI Laifu;LIU Yingjuan(School of Life Science and Technology,Nanyang Normal University,Nanyang 473061,Henan,China)

机构地区：[1]南阳师范学院生命科学与技术学院

出　　处：《陕西师范大学学报（自然科学版）》2020年第1期99-105,共7页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：国家自然科学基金青年项目(31501865)

摘　　要：为了探讨长期社会击败应激对小家鼠行为的影响及其神经内分泌机制,采用“居留-入侵”模型对小家鼠实施连续10 d的社会击败应激;通过旷场、社会接近回避和悬尾三种行为学方法筛选易感和非易感小家鼠;通过免疫组织化学的方法研究易感及非易感小家鼠在面对攻击鼠时,边缘系统脑区即刻早期基因c-Fos表达变化的差异;采用酶联免疫吸附测定法比较易感、非易感小家鼠血清皮质酮含量的差别。结果表明:只有39.1%的小家鼠为应激易感个体;易感、非易感及对照组小家鼠的c-Fos表达在前额叶皮质、下丘脑室旁核、杏仁核及外侧缰核等脑区表现出较大差异;易感小家鼠血清皮质酮含量显著高于非易感及对照组小家鼠。基于此,小家鼠在面对社会击败应激时可表现为易感和非易感两种类型,前额叶皮质、下丘脑室旁核、杏仁核以及外侧缰核等相关脑区的活动及HPA轴敏感性的差异可能在上述两种类型中起着重要的调控作用。The purpose of this study was to investigate the effects of chronic social defeat stress(CSDS)on the behavior of mice and the underlying neuroendocrine mechanisms.The CSDS was carried out by"residence-intruder"model,in which the subjects(descendants of wild mice)were exposed to a larger group of aggressive Kunming male mouse for 10 min in 10 successive days.Open field test,social approach and avoidance test as well as tail suspension test were used to screen the susceptible and unsusceptible mouse.c-Fos expression differences were studied in the limbic brain areas among susceptible,unsusceptible and control groups.The serum corticosterone levels were studied by ELISA.Results showed that:only 39.1%of mouse showed depression-like behaviors when were exposed to CSDS;c-Fos expressions in the prefrontal cortex,hypothalamus paraventricular nucleus,medial amygdala and lateral habenular nucleus were significantly different among the three groups;serum corticosterone levels were significantly higher in susceptible group than those in the other two groups.The results indicated that the subjects could be divided into susceptible and unsusceptible subtypes when they were exposed to CSDS.The different sensitivity of HPA axis and activities in some brain areas of limbic system such as prefrontal cortex,hypothalamic paraventricular nucleus,amygdala and lateral habenular nucleus may play important regulation roles in these two subtypes.

关 键 词：社会击败 小家鼠 应激 C-FOS 皮质酮 

分 类 号：R944.9[医药卫生—药剂学]