18β-甘草次酸钠对变应性鼻炎大鼠鼻黏膜NF-κBp50及血清IL-4表达的影响  

Effects of 18beta-solidum glycrrhetinic acid on nuclear factor-kappaBp50 of nasal mucosa and IL-4 of serum in rats with allergic rhinitis

作　　者：李丽[1] 王有虎[1] 侯赟[1] 桂岩[1] 陈小婉[1] 陈康兵[1] 席克虎[1] 马春霞[1] 刘小涵 张小兵[1] LI Li;WANG You-hu;HOU Yun;GUI Yan;CHEN Xiao-wan;CHEN Kang-bing;XI Ke-hu;MA Chun-xia;LIU Xiao-han;ZHANG Xiao-bing(Department of Otolaryngology Head and Neck Surgery,The First Hospital of Lanzhou University,Lanzhou 730030;Lanzhou University,Lanzhou 730000,China)

机构地区：[1]兰州大学第一医院耳鼻咽喉头颈外科

出　　处：《西安交通大学学报（医学版）》2020年第1期58-63,共6页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金地区基金项目(No.81160449)~~

摘　　要：目的观察18β-甘草次酸钠(18beta-solidum glycrrhetinic acid,18β-SGA)对变应性鼻炎(allergic rhinitis,AR)大鼠鼻黏膜中NF-κBp50及血清中白介素-4(IL-4)表达的影响。方法卵清蛋白致敏Wistar大鼠建立AR动物模型。造模成功后,将大鼠随机分为正常对照组、AR模型组、布地奈德组和18β-SGA低剂量(20 mg/kg)、高剂量(40 mg/kg)干预组,分别在给药干预2周和4周后取大鼠鼻黏膜和血清,免疫组化法观察NF-κBp50在大鼠鼻黏膜的分布,RT-qPCR和Western blot检测NF-κBp50 mRNA和蛋白相对表达量,酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)检测血清中OVA特异性sIgE(OVA-sIgE)及IL-4的表达。结果大鼠鼻黏膜中NF-κBp50主要表达在鼻黏膜假复层纤毛柱状上皮细胞、固有层的腺体细胞、血管内皮细胞、炎性细胞的细胞质、细胞核中。AR组大鼠较正常大鼠鼻黏膜中NF-κBp50 mRNA及蛋白的表达量增加(P<0.05),经18β-SGA或布地奈德干预后,其表达量减少(P<0.05)。AR组大鼠血清中OVA-sIgE、IL-4表达量较对照组升高(P<0.05),经18β-SGA或布地奈德干预后,IL-4表达量下降(P<0.05)。结论18β-SGA可能通过抑制NF-κB信号通路活性,降低NF-κBp50表达,下调血清IL-4水平,从而发挥抗炎作用。Objective To investigate the effects of 18beta-solidum glycrrhetinic acid(18β-SGA)on the expression of nuclear factor-kappaBp50(NF-κBp50)in nasal mucosa and interleukin-4(IL-4)in serum of allergic rhinitis(AR)rats.Methods Wistar rats were sensitized with ovalbumin(OVA)to establish an animal model of AR.After successful modeling,the rats were randomly divided into control group,model group,budesonide group and 18β-SGA different dose treatment groups(20 mg/kg group and 40 mg/kg group).The nasal mucosa and serum was collected at 2 weeks and 4 weeks after administration.The distribution of NF-κBp50 in nasal mucosa was observed by immunohistochemistry.RT-qPCR and Western blot were used to detect NF-κBp50 expression.ELISA was used to detect OVA-specific immunoglobulin E(OVA-sIgE)and IL-4 expression in serum.Results The results of immunohistochemistry showed that NF-κBp50 was mainly expressed in nasal mucosa s cytoplasm and nucleus of pseudostratified ciliated columnar epithelium,vascular endothelial cells in the lamina propria,glandular cells and inflammatory cells.RT-qPCR and Western blot analysis showed that the relative expressions of NF-κBp50 mRNA and protein in nasal mucosa of AR rats were increased compared with those in normal rats(P<0.05),but they were decreased after treatment with 18β-SGA or budesonide(P<0.05).ELISA showed that the expressions of OVA-sIgE and IL-4 in serum of AR rats were increased compared with those in normal rats(P<0.05),but the expression of IL-4 in AR rats decreased after treatment with 18β-SGA or budesonide(P<0.05).Conclusion 18β-SGA can significantly inhibit the activity of NF-κB,and decrease the expressions of NF-κBp50 and IL-4.Therefore,it has anit-inflammatory action and may be used in the treatment of AR.

关 键 词：18β-甘草次酸钠 变应性鼻炎 NF-ΚBP50 白介素-4 

分 类 号：R856.76[医药卫生—航空、航天与航海医学]