Ac-SDKP对炎性因子MRP-14(S100A9)的调节在硅肺大鼠纤维化病变中的作用  

The role of N-acetyl-seryl-aspartyl-lysyl-proline in the regulation of inflammatory factor MRP-14 (S100A9) in silicosis rats

作　　者：邓海静 王瑾 侯晓丽 高学敏 杨方 DENG Hai-jing;WANG Jin;HOU Xiao-li;GAO Xue-min;YANG Fang(School of Basic Medical Sciences,North China University of Science and Technology,Hebei Key Laboratory for Chronic Diseases,Tangshan Key Laboratory for Preclinical and Basic Research on Chronic Diseases,Tangshan 063000,China)

机构地区：[1]华北理工大学基础医学院河北省慢性疾病重点实验室唐山市慢性病临床基础研究重点实验室

出　　处：《西安交通大学学报（医学版）》2020年第1期69-73,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金项目(No.81302395);河北省高等学校科学技术研究青年项目(No.QN2016147);华北理工大学博士科研启动项目;华北理工大学杰出青年基金项目;河北省自然科学基金培育基金~~

摘　　要：目的探讨N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸(Ac-SDKP)是否通过抑制炎性因子髓系相关蛋白14(myeloid-related protein14,MRP-14)以及胶原蛋白的表达,从而阻抑硅肺大鼠纤维化病变中硅结节的形成和胶原蛋白的沉积。方法采用一次性支气管灌注二氧化硅(SiO 2)粉尘的方法制备大鼠硅肺动物模型,60只SPF级健康成年大鼠随机分为对照4周组、对照8周组、硅肺模型4周组、硅肺模型8周组、Ac-SDKP预防治疗组和Ac-SDKP抗纤维化治疗组,每组10只。免疫组织化学技术检测MRP-14蛋白在硅肺模型组织内的定位表达;Western blot法检测MRP-14以及胶原蛋白在硅肺模型组织内的表达。结果与相应的对照组相比,硅肺模型组大鼠硅肺纤维化区域MRP-14和胶原蛋白的表达增强,差异具有统计学意义(P<0.05)。与硅肺模型8周组相比,给予Ac-SDKP干预后MRP-14和胶原蛋白表达明显降低,差异具有统计学意义(P<0.05)。结论Ac-SDKP可通过抑制炎性因子MRP-14的表达而发挥其抗硅肺纤维化的作用。Objective To explore whether N-acetyl-seryl-aspartyl-lysyl-proline(Ac-SDKP)inhibits the formation of silica nodules and the deposition of collagen by inhibiting the expressions of inflammatory factor myeloid-related protein14(MRP-14)and collagen in silicosis rats.Methods The silicosis model of rats was established by one-off bronchial instillation of silicon(SiO 2)dust.Sixty healthy adult rats of SPF grade were randomly divided into six groups:control 4-week group,control 8-week group,silicosis model 4-week group,silicosis model 8-week group,Ac-SDKP prevention treatment group,and Ac-SDKP anti-fibrosis treatment group,with ten rats in each group.Immunohistochemical method was used to detect the expression of MRP-14 protein in silicosis model tissues.Western blot method was used to detect the expressions of MRP-14 and collagen protein in silicosis model tissues.Results Compared with those in the corresponding control group,the expressions of MRP-14 and collagen in silicosis fibrosis areas were significantly increased in the dust-exposed group(P<0.05).Compared with those in the silicosis model 8-week group,the expressions of MRP-14 and collagen were significantly decreased after Ac-SDKP intervention(P<0.05).Conclusion Ac-SDKP can inhibit silicosis in rats by inhibiting the regulation of MRP-14 expression.

关 键 词：N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸(Ac-SDKP) 髓系相关蛋白14 胶原蛋白 硅结节 硅肺纤维化 

分 类 号：R329.21[医药卫生—人体解剖和组织胚胎学]