复方芪藻汤调控Foxo3a/Bim抑制胃溃疡大鼠胃黏膜上皮细胞凋亡及其机制  

Fufangqizao decoction regulates Foxo3a/Bim inhibiting apoptosis of gastric mucosal epithelial cells in rats with experimental gastric ulcer

作　　者：陈畅 隋璐[1] 赵春莹[1] CHEN Chang;SUI Lu;ZHAO Chun-ying(Shenyang Medical College,Shenyang 110034,China)

机构地区：[1]沈阳医学院

出　　处：《西安交通大学学报（医学版）》2020年第1期145-149,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：辽宁省自然科学基金资助项目(No.2014020129);辽宁省大学生科研创新项目(No.201410164000004);沈阳医学院大学生科研创新项目(No.20141012)~~

摘　　要：目的探讨复方芪藻汤治疗胃溃疡的可能机制。方法60只大鼠随机分为正常对照组、溃疡模型组及复方芪藻汤低、中、高剂量组(3、6、12 g/kg)和奥美拉唑(药物对照)组,每组10只。采用乙酸烧灼法制备大鼠胃溃疡模型;Western blot技术检测胃溃疡组织Foxo3a、Bim蛋白的表达,TUNEL染色检测胃黏膜上皮细胞凋亡。结果与溃疡模型组相比,复方芪藻汤各剂量组与奥美拉唑组Foxo3a、Bim表达水平明显降低,且复方芪藻汤高剂量组较奥美拉唑组更为显著(P<0.05)。复方芪藻汤各剂量组凋亡指数分别为(26.79±2.54)%、(22.41±3.67)%、(14.38±3.40)%,较溃疡模型组[(30.75±2.93)%]明显下降(P<0.05)。结论复方芪藻汤可能通过下调Foxo3a/Bim表达而抑制胃黏膜上皮细胞凋亡,发挥其抗胃溃疡作用。Objective To explore the possible mechanism of Fufangqizao Decoction in the treatment of gastric ulcer.Methods Sixty rats were randomly divided into normal control group,ulcer control group,Fufangqizao decoction low-,medium-and high-dose groups(3 g/kg,6 g/kg,and 12 g/kg);omeprazole group,with 10 in each group.Rat gastric ulcer model was prepared by acetic acid ablation method.The protein expressions of Foxo3a and Bim in gastric ulcer were detected by Western blot.Apoptosis of gastric mucosal epithelial cells was detected by TUNEL staining.Results Compared with those in ulcer control group,the expression levels of Foxo3a and Bim in Fufangqizao decoction groups and omeprazole group were significantly lower,and the effect of high-dose Fufangqizao decoction on the expressions of Foxo3a and Bim was more significant than that of omeprazole(P<0.05).The apoptosis index in each group was(26.79±2.54)%,(22.41±3.67)%,and(14.38±3.40)%,which were significantly lower than that in ulcer model group(30.75±2.93)%(P<0.05).Conclusion Fufangqizao decoction may inhibit gastric mucosal epithelial cell apoptosis by down-regulating Foxo3a/Bim expression and thus exerts its anti-gastric ulcer effect.

关 键 词：复方芪藻汤 胃溃疡 细胞凋亡 FOXO3A BIM 

分 类 号：R285[医药卫生—中药学]