受激辐射耗尽超分辨显微成像的研究进展及应用  

Research Progress and Application of Stimulated Emission Depletion Super-Resolution Imaging

作　　者：阮贺飞 吴亚运 袁景和[1] 石彦 方晓红[1] RUAN He-fei;WU Ya-yun;YUAN Jing-he;SHI Yan;FANG Xiao-hong(Beijing National Research Center for Molecular Sciences,Key Laboratory of Molecular Nanostructure and Nanotechnology,Institute of Chemistry,Chinese Academy of Sciences,Beijing 100190;School of Medicine,Tsinghua-Peking Joint Center for Life Sciences,Tsinghua University,Beijing 100084)

机构地区：[1]中国科学院化学研究所,分子纳米结构与纳米技术重点实验室,北京分子科学国家研究中心,北京100190 [2]清华大学医学院,生命科学联合中心,北京100084

出　　处：《分析科学学报》2019年第6期723-731,共9页Journal of Analytical Science

基　　金：国家自然科学基金重点项目(No.21735006)

摘　　要：荧光显微镜凭借其非接触、无损伤、可实时探测生物样品内部等优点,成为生命科学研究中不可或缺的工具,但是Abbe光学衍射极限的存在限制了其对亚细胞尺度生化过程的进一步研究。作为突破光学衍射极限的远场光学显微镜,受激辐射耗尽(Stimulated Emission Depletion,STED)超分辨荧光显微术,因其超高时空分辨率和三维层析能力,成为备受关注的新型成像和分析表征工具。本文结合我们课题组在STED显微成像研究方面的工作,综述了近年来STED显微成像技术的研究进展及其在细胞成像中的应用。Fluorescence microscopy is an indispensable tool in life science research because of its non-contact,non-invasive,real-time detection of the interior of biological samples,but the existence of E.Abbe’s optical diffraction limit hinders the further study of subcellular physiological processes.As the earliest far-field optical microscopy that broke the optical diffraction limit,stimulated emission depletion(STED) super-resolution microscopy has become a new imaging tool which attracts great attention due to its ultra-high spatial-temporal resolution and three-dimensional tomographic capability.Based on our experience in the development and application of STED microscope,this paper reviews the recent advances in STED microscopy and its application in cell imaging.

关 键 词：受激辐射耗尽显微镜 超分辨成像 荧光探针 细胞成像 衍射极限 

分 类 号：O657.3[理学—分析化学]