无血预充大鼠外周静脉-动脉体外膜氧合模型建立的初步探索  

Establishment of veno-arterial extracorporeal membrane oxygenation model in rats without blood priming

作　　者：蔡黎明 张巧妮 李勇男[1] 黄永胡 吉冰洋[1] Cai Liming;Zhang Qiaoni;Li Yongnan;Huang Yonghu;Ji Bingyang(Department of Cardiopulmonary Bypass,State Key Laboratory of Cardiovascular Diseases,Fuwai Hospital,National Center for Cardiovascular Disease,Chinese Academy of Medical Science and Peking Union Medical College,Beijing 100037,China;不详)

机构地区：[1]北京中国医学科学院北京协和医学院国家心血管病中心阜外医院体外循环中心,北京100037 [2]北京中国医学科学院北京协和医学院心血管疾病国家重点实验室,北京100037 [3]首都儿科研究所附属儿童医院,北京100020

出　　处：《中国体外循环杂志》2019年第6期360-363,共4页Chinese Journal of Extracorporeal Circulation

基　　金：国家自然科学基金面上项目(81470578)

摘　　要：目的建立无血预充的大鼠静脉-动脉体外膜氧合(V-A ECMO)模型,为进一步探讨和研究ECMO期间的器官损伤机制及相应保护策略等问题奠定基础。方法随机选取5只成年SD雄性大鼠,七氟烷诱导麻醉,颈静脉置入自制多孔静脉引流管作为静脉引流端,左侧股动脉置入22 G一次性使用静脉留置针监测血压,右侧股动脉置入同款留置针为动脉灌注端。从监测端动脉通路给予肝素(500 U/kg)抗凝,活化凝血时间达400 s以上开始ECMO运转,2 h后停止运转。分别于术前,术中1 h,2 h三个时间点抽取动脉血气分析,记录各时间点血气值,并于术中实时监测记录动脉压及心率。结果所有大鼠均顺利完成实验,并在撤除ECMO后于麻醉状态下处死。各时间点血气结果平稳。术中心率在300次/min左右,无恶性心律失常,血压平稳,平均动脉压维持在70~90 mm Hg。结论本实验成功建立大鼠外周V-A ECMO模型,术前无需血液预充,术中麻醉深度精准可控、呼吸循环稳定、血气结果满意,操作简便易行,成功率高,可用于研究ECMO期间血流动力学改变,同时使脏器损伤的机制探讨及干预治疗的靶点研究成为可能。Objective Extracorporeal membrane oxygenation(ECMO)causes many complications,but the underlying mechanisms are unclear.The purpose of this study was to establish an veno-arterial ECMO model in rats without blood priming,so as to lay a foundation for further discussion and research on mechanisms of organ injury and corresponding protective strategies during ECMO.Methods A total of 6 adult SD male rats were anesthetized with sevoflurane,and a homemade catheter was inserted into the right jugular vein for venous drainage.The left femoral artery was implanted with a 22 G catheter to monitor arterial blood pressure,and the right femoral artery was implanted with a 22 G catheter as the arterial perfusion port.Heparin(500 U/kg)was given to the left femoral artery for anticoagulation,and the ACT reached more than 400 s to start the transfer.The transfer was weaned after 2 h.Blood gas analysis was measured at three time points(pre-operatively,1 h peri-operatively,pre-weaned).Arterial pressure and heart rate were monitored continuously.Results All the 6 rats completed the experiment successfully and were sacrificed under anesthesia after weaning.Conclusion This successful ECMO rats model with no blood primed preoperatively was characterized by precise control of anesthetic depth,high success rate and easy operation.It could be used in study of organ dysfunction caused by ECMO.

关 键 词：大鼠 体外膜氧合 动物模型 体外循环 生命支持 

分 类 号：R47[医药卫生—护理学]