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作 者:王乐旬 吴惠娟 张盛昔 荣向路 郭姣 WANG Lexun;WU Huijuan;ZHANG Shengxi;RONG Xianglu;GUO Jiao(Guangdong Metabolic Disease Research Center of Integrated Chinese and Western Medicine,Guangdong Key Laboratory of Metabolic Disease Prevention and Treatment of Traditional Chinese Medicine,Joint Laboratory of Guangdong,Hong Kong and Macao on Glycolipid Metabolic Diseases,Institute of Chinese Medicine Sciences,Guangdong Pharmaceutical University,Guangzhou 510006,China)
机构地区:[1]广东省代谢病中西医结合研究中心/广东省代谢性疾病中医药防治重点实验室/粤港澳联合代谢病重点实验室/广东药科大学中医药研究院
出 处:《广东药科大学学报》2019年第6期763-767,共5页Journal of Guangdong Pharmaceutical University
基 金:国家自然科学基金重点项目(81530102);广东省自然科学基金博士启动纵向协同项目(2018A030310403);广东省医学科学技术研究基金(A2018068);广东药科大学创新强校工程资助项目(2018KTSCX112)
摘 要:目的探讨不同建模方法对链脲佐菌素(streptozotocin,STZ)诱导1型糖尿病模型成模率的影响。方法选择8~10周龄雄性C57BL/6小鼠,随机分为对照组注射柠檬酸盐缓冲液,实验组一连续5 d腹腔注射STZ(50 mg/kg,每天1次),实验组二前4 d腹腔注射STZ(50 mg/kg)+第5天腹腔注射STZ(100 mg/kg)、实验组三饥饿12 h后给予腹腔注射STZ(50 mg/kg,每天1次,连续5 d)。末次注射后,每天观察小鼠摄食和饮水情况,每周测定小鼠随机血糖和体质量,连续4周。实验结束后取胰腺应用免疫组化观察胰岛情况。结果和对照组相比,3组小鼠的饮水量和摄食量在STZ注射后都显著增加,且实验组二的饮水量比实验组一和实验组三的显著升高。和对照组相比,3组小鼠的体质量逐渐下降。三组的随机血糖在STZ注射后第1周开始升高,一直维持到第4周,实验组二的血糖升高最为明显。实验组二从第2周开始,成模率91.7%,第4周的成模率为100%;实验组一的成模率第2周为50%,第4周的成模率为83.3%;实验组三第2周的成模率为36.4%,第4周的成模率为63.6%。胰腺免疫组化结果显示3组的胰岛面积均显著减小。结论连续4 d小剂量腹腔注射STZ(50 mg/kg)+第5天大剂量腹腔注射STZ(100 mg/kg)是较理想的建立1型糖尿病模型的方法。Objective To explore the effect of different construction methods on streptozotocin-induced type 1 diabetes mellitus model.Methods Adult male C57 BL/6 mice were randomly divided into the control group injected with citric acid salt buffer,the experimental group 1 injected i.p.with STZ(50 mg/kg)for 5 consecutive days,the experimental group 2 injected i.p.with STZ(50 mg/kg)for 4 consecutive days and 100 mg/kg STZ for the fifth day,and the experimental group 3 fasted for 12 h and then injected i.p.with STZ(50 mg/kg)for 5 consecutive days.The general appearance,body mass,blood glucose level,and pathological damage of pancreas were observed.Results Compared with the control group,the water and food intake in three experimental groups were significantly increased after STZ injection,and the water intake in group 2 was significantly higher than that of group 1 and 3.The body weight was markedly decreased in three experimental groups.The random blood glucose was significantly increased in three experimental groups after STZ injection from the first week to the fourth week.The modeling rate was 100%in experimental group 2,83.3%in experimental group 1 and 63.6%in experimental group 3,respectively.The immunohistochemistry showed that the islets were significantly reduced in three experimental groups.Conclusion Intraperitoneal low-dose STZ(50 mg/kg)injection for 4 consecutive days and high-dose STZ(100 mg/kg)for the fifth day is an optimal method for establishing type 1 diabetes mellitus model.
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