阿奇霉素-苦木注射液联用对炎症细胞因子释放影响的体外研究  

In vitro Effect of Azithromycin-Kumu Injection on Release of Inflammatory Cytokines

作　　者：张杰[1] 李乔 郭文娜 许晓蒙 孙发鑫 许卉[1] ZHANG Jie;LI Qiao;GUO Wen-na;XU Xiao-meng;SUN Fa-xin;XU Hui(School of Pharmacy,Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong,Key Laboratory of Molecular Pharmacology and Drug Evaluation(Yantai University),Ministry of Education,Yantai University,Yantai 264005,China)

机构地区：[1]烟台大学新型制剂与生物技术药物研究山东省高校协同创新中心分子药理和药物评价教育部重点实验室(烟台大学)

出　　处：《烟台大学学报（自然科学与工程版）》2020年第1期115-119,共5页Journal of Yantai University(Natural Science and Engineering Edition)

基　　金：山东省自然科学基金资助项目(ZR2017MH061);创新天然药物与中药注射剂国家重点实验室开放课题(QFSKL2018007)

摘　　要：实验研究阿奇霉素与苦木注射液体外联合用药对前炎症因子NO、TNF-α抑制作用的影响.采用脂多糖(LPS)体外刺激小鼠单核巨噬细胞RAW264.7,刺激细胞释放TNF-α、NO前炎性因子.采用MTT法评价细胞毒性;Griess法检测培养液上清中NO含量;ELISA试剂盒法检测TNF-α含量;金正均法计算Q值进行药效联用评价.结果表明阿奇霉素联合苦木注射液在10~200μg/mL浓度范围内可明显抑制LPS诱导巨噬细胞RAW264.7释放的炎性因子TNF-α、NO,并呈现良好的剂量依赖关系.阿奇霉素联合苦木注射液对炎症因子NO具相加抑制作用,对炎症因子TNF-α具协同抑制作用.The aim of this study is to evaluate the in vitro inhibitory effects of pro-inflammatory cytokines nitric oxide(NO)and tumor necrosis factor-α(TNF-α)using azithromycin combined with Kumu injection.Mouse macrophage RAW264.7 cell is stimulated by lipopolysaccharide(LPS)to induce pro-inflammatory cytokines TNF-αand NO.MTT assay is used to evaluate the cytotoxicity.The production of NO is determined using Griess assay.The content of TNF-αis determined using ELISA method.The Q value is calculated using JinZhenjun’s method to evaluate pharmacodynamic interaction(Q^0.85-1.15 for additive effects,Q≥1.15 for synergism,Q≤0.85 for antagonism).The results show that this combination can inhibit the inflammatory factors TNF-αand NO in the concentration range of 10-200μg/mL with a good dose-dependent relationship.In conclusion,azithromycin combined with Kumu injection has synergistic activity against inflammatory factor TNF-αand additive activity against inflammatory factor NO.

关 键 词：阿奇霉素 苦木注射液 联合用药 巨噬细胞RAW264.7 TNF-Α NO 

分 类 号：R917.2[医药卫生—药物分析学]