益糖康治疗糖尿病气阴两虚证血清代谢组学研究  

Study on Serum Metabolomics of Diabetes Mellitus with Qi and Yin Deficiency Syndrome Based on UPLC/Q-TOF-MS Combined Technology

作　　者：杨宇峰[1] 石岩[1] YANG Yufeng;SHI Yan(Liaoning Universtity of Traditional Chinese Medicine,Shenyang Liaoning China 110847)

机构地区：[1]辽宁中医药大学

出　　处：《中医学报》2019年第12期2593-2596,共4页Acta Chinese Medicine

基　　金：沈阳市科学技术计划项目(18-013-0-82);辽宁省“兴辽英才计划”青年拔尖人才资助项目(XLYC1807145);辽宁省教育厅科学研究项目(20171109)

摘　　要：目的:运用代谢组学方法研究中药益糖康对于糖尿病气阴两虚证大鼠的干预作用,探讨其在体内发挥药效的作用机制。方法:通过超高效液相色谱与串联四级杆飞行时间质谱仪(ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry,UPLC/Q-TOF-MS)联用技术分析药物作用后大鼠血清的代谢轨迹,采用主成分分析(PCA)和偏最小二乘方判别分析(PLS-DA)寻找各组代谢物差异,筛选潜在内源性标志物。结果:初步鉴定出可能的8种潜在代谢生物标志物,分别为乳酸、1,5脱水山梨醇、L-苯丙氨酸、L-精氨酸、牛磺酸、棕榈酸、花生四烯酸、L-苹果酸。这些内源性标志物在益糖康治疗组恢复明显。结论:中药益糖康可通过调节糖代谢、脂肪代谢、氨基酸代谢、三羧酸循环等途径来发挥对机体糖稳态的调控。本研究从体内代谢角度揭示了益糖康对糖尿病气阴两虚证治疗的作用机制。Objective:To study the therapeutic effect of Yi Tang Kang on Qi and Yin Deficiency Syndrome in Diabetic Rats by Metabolomics and explore its mechanism of action in vivo.Methods:The metabolic trajectories of serum were measured by UPLC/Q-TOF-MS.The differences of metabolites were observed with PCA and PLS-DA and the potential endogenous markers were screened.Results:Eight potential metabolic biomarkers were identified and these endogenous markers were found to be effective in the treatment group.Conclusion:Chinese medicine Yi Tang Kang can regulate the regulation of sugar steady state by regulating glucose metabolism,fat metabolism,amino acid metabolism and tricarboxylic acid cycle.The study revealed the mechanism of traditional Chinese medicine Yi Tang Kang on the treatment of diabetes mellitus with Qi and Yin deficiency Syndrome.

关 键 词：糖尿病 气阴两虚证 益糖康 血清代谢组学 通路分析 

分 类 号：R285.5[医药卫生—中药学]