lncRNA SBF2-AS1通过调控miR-140-5p/VEGFA分子轴促进宫颈癌HeLa细胞上皮间质转化  

lncRNA SBF2-AS1 promotes epithelial-mesenchymal transition of cervical cancer cells via regulating miR-140-5p/VEGFA axis

作　　者：方淑芬[1] 熊树华[2] 黄欧平[1] 万玉珍[1] FANG Shufen;XIONG Shuhua;HUANG Ouping;WAN Yuzhen(Department of Gynaecology,Maternal and Child Health Center of Jiangxi Province,Nanchang 330006,Jiangxi,China;Department of Oncology,Maternal and Child Health Center of Jiangxi Province,Nanchang 330006,Jiangxi,China)

机构地区：[1]江西省妇幼保健院妇科,江西南昌330006 [2]江西省妇幼保健院肿瘤科,江西南昌330006

出　　处：《中国肿瘤生物治疗杂志》2019年第12期1331-1336,共6页Chinese Journal of Cancer Biotherapy

摘　　要：目的:探讨1ncRNASBF2-AS1通过调控miR-140-5p/.血管内皮生长因子A(VEGFA)分子轴对宫颈癌HeLa细胞上皮间质转化(EMT)的影响。方法:细胞培养和转染后分为NC、miR-140-5p mimic、miR-140-5p mimic+pcDNA-VEGFA、si-lncRNA SBF2-AS1+pcDNA-VEGFA及si-lncRNA SBF2-AS 1+miR-140-5p mimic组5组。采用qPCR检测1ncRNA SBF2-AS1在宫颈癌组织及细胞系中的表达水平,双荧光素酶报告基因验让1ncRNASBF2-AS1、miR-140-5p与VEGFA的靶向关系,WB检测HeLa细胞中VEGFA及EMT标志物N-cadherin、Vimentin和E-cadherin的表达水平,Transwell实验检测HeLa细胞侵袭和迁移能力。结果:1ncRNA SBF2-AS 1在宫颈癌组织及细胞系中高表达(P<0.05或P<0.01),1ncRNA SBF2-AS 1靶向结合miR-140-5p,且VEGFA是miR-140-5p的靶基因(P<0.05)。敲降lncRNA SBF2-AS1抑制HeLa细胞侵袭、迁移及EMT。进一步实验证实,1ncRNA SBF2-AS1通过miR-140-5p上调VEGFA的表达水平,从而促进HeLa细胞侵袭、迁移及EMT(P<0.05或P<0.01)。结论:1ncRNA SBF2-AS1通过miR-140-5p/VEGFA分子轴促进HeLa细胞EMT。Objective:To investigate the effect of IncRNA SBF2-AS1 on epithelial-mesenchymal transition(EMT) of cervical cancer HeLa cell via regulating miR-140-5 p/VEGFA(vascular endothelial growth factor A) axis.Methods:After cell culture and transfection,the cells were divided into 5 groups:NC group,miR-140-5 p mimic group,miR-140-5 p mimic+pcDNA-VEGFA group,si-lncRNA SBF2-AS1+pcDNA-VEGFA group and si-lncRNA SBF2-AS1+miR-140-5 p mimic group.The expression level of lncRNA SBF2-AS1 in cervical cancer tissues and cell lines was detected by qPCR.The targeted relationship between lncRNA SBF2-AS1,miR-140-5 p and VEGFA was confirmed by Dual luciferase reporter gene assay.The expression levels of VEGFA and EMT-related proteins N-cadherin,Vimentin and E-cadherin in HeLa cells were detected by WB.The invasion and migration of HeLa cells were detected by Transwell.Results:lncRNA SBF2-AS1 was highly expressed in cervical cancer tissues and cell lines(P<0.05 or P<0.01).Dual luciferase reporter gene assay confirmed that IncRNA SBF2-AS1 targetedly combined with miR-140-5 p and VEGFA was a target gene of miR-140-5 p(P<0.05).Knockdown of IncRNA SBF2-AS1 inhibited invasion and migration as well as EMT of HeLa cells.Further experiment confirmed that IncRNA SBF2-AS1 up-regulated the expression level of VEGFA via miR-140-5 p,thereby promoting invasion,migration and EMT of HeLa cells.Conclusion:lncRNA SBF2-AS1 promotes EMT of HeLa cells via miR-140-5 p/VEGFA axis.

关 键 词：lncRNA SBF2-AS1 miR-140-5p 血管内皮生长因子A 宫颈癌 HeLa细胞 上皮间质转化 

分 类 号：R392.12[医药卫生—免疫学] R737.33[医药卫生—基础医学]