基于不同煎煮配伍法探讨关木通配伍干姜的减毒机制  

Study on the attenuation mechanism of aristolochia manshuriensis compatibility with zingiberis rhizoma based on different methods of boiling and blending

作　　者：张月婵[1] 邵珠莹 韩怡[1] 王建春[1] 施怡 Zhang Yuechan;Shao Zhuying;Han Yi;Wang Jianchun;Shi Yi(Department of Pharmacy,Zhangjiagang Hospital of Traditional Chinese Medicine,Affiliated Hospital of Nanjing University of Chinese Medicine,Graduate Workstation of Enterprises in Jiangsu Province,Zhangjiagang 215600,China;Department of Pharmacy,Suzhou Science&Technology Town Hospital,Suzhou 215000,China)

机构地区：[1]南京中医药大学附属张家港医院药学部,江苏省研究生工作站,张家港215600 [2]江苏省苏州科技城医院药学部,215000

出　　处：《国际中医中药杂志》2019年第12期1347-1352,共6页International Journal of Traditional Chinese Medicine

基　　金：苏州市科技计划项目(SYSD2015162)。

摘　　要：目的比较干姜、关木通不同煎煮方法对大鼠急性肾功能损伤的影响,并在体外观察其对正常人肾小管上皮细胞(human kidney cell-2,HK-2)的影响,探讨关木通配伍干姜的减毒机制。方法将大鼠按随机数字表法分为空白组、阳性对照组、关木通组、合煎组、分煎组,每组8只。采用生理盐水灌胃法制备水负荷大鼠模型。各给药组灌胃相应药物后,收集大鼠尿液,测定24 h尿量。将大鼠按随机数字表法分为空白组、关木通组、合煎组、分煎组,每组8只。各给药组灌胃相应药物2周后,采用7600P全自动生化分析仪检测大鼠血清BUN、SCr水平及尿液中尿肌酐(urine creatinine,UCr)、尿蛋白(urinary protein,PRO)水平,采用HE染色观察各组大鼠肾组织病理学改变。体外培养HK-2细胞,并分为对照组、关木通组、合煎组、分煎组,相应药物干预24 h后,采用CCK-8法检测细胞存活率,赫斯特染色(Hoechst)法观察细胞凋亡情况。结果各组大鼠24 h排尿量比较差异无统计学意义(P>0.05);与关木通组比较,合煎组大鼠肾脏系数[(0.0101±0.0058)比(0.0133±0.0078)],血清SCr[(38.52±0.58)μmol/L比(46.61±0.72)μmol/L]、BUN[(8.55±0.12)mmol/L比(10.21±0.30)mmol/L],尿液UCr[(52.21±0.89)μmol/L比(57.71±0.67)μmol/L]、PRO[(29.89±0.18)mg/L比(34.23±6.05)mg/L]水平降低(P<0.05);合煎组HK-2细胞存活率[(72.45±3.70)%比(55.92±8.39)%]升高(P<0.01),细胞凋亡率[(7.9±2.6)%比(31.6±9.1)%]降低(P<0.01)。结论采用传统的合并煎煮法干姜配伍关木通可达到一定的减毒作用,而采用分煎合用法煎煮的配伍方法不能达到减毒作用。Objective Comparison of effects of Zingiberis Rhizoma and Aristolochia manshuriensis on diuretic effect and acute renal injury in rats by combining two methods of co-decoction and mixed-decoction.The effects of in vitro observation on normal human renal tubular epithelial cells(HK-2)were observed.Methods The rats were randomly divided into five groups:blank group,positive control group,aristolochia manshuriensis group,combined decoction group and divided decoction group,8 rats in each group.The water-loading rat model was established by intragastric administration of normal saline.The urine of rats was collected and the volumes of urine were measured for 24 hours after the corresponding drugs were given to each group.After 2 weeks of gavage of the corresponding drugs in each group,the serum BUN,SCr and urine UCr and PRO levels were measured by 7600P automatic biochemical analyzer,and renal histopathology were observed by HE staining.The HK-2 cells were cultured in vitro and divided into control group,Aristolochia manshuriensis group,mixed decoction group and sub-decoction group.After 24 hour intervention,the activity of cells was detected by CCK-8 method and the apoptosis was observed by Hoechst stain method.Results There was no significant difference in 24 hour urine output between the groups(P>0.05).Compared with Aristolochia manshuriensis group,the kidney coefficient(0.0101±0.0058 vs.0.0133±0.0078),SCr(38.52±0.58μmol/L vs.46.61±0.72μmol/L),BUN(8.55±0.12 mmol/L vs.10.21±0.30 mmol/L),UCr(52.21±0.89μmol/L vs.57.71±0.67μmol/L),PRO(29.89±0.18 mg/L vs.34.23±6.05 mg/L)of combined decoction group significantly decreased(P<0.05).The survival rate of HK-2 cells(72.45%±3.70%vs.55.92%±8.39%)in combined decoction group significantly increased(P<0.01),and the apoptosis rate(7.9%±2.6%vs.31.6%±9.1%)significantly decreased(P<0.01).Conclusions The traditional co-decoction method of Aristolochia manshuriensis compatibility with Zingiberis Rhizoma can achieve a certain attenuation effect,and the mixed-decoction

关 键 词：关木通 干姜 中药配伍 煎煮法 中药毒性 急性肾损伤 大鼠 

分 类 号：R28[医药卫生—中药学]