基于网络药理学的三七治疗和改善瘢痕增生分子机制研究  

Treatment and improvement of scar hyperplasia by panax notoginseng based on network pharmacology study on molecular mechanisms

作　　者：黄敬文[1] 韩雪 赵旭[1] 李续博[1] 董兴中[1] 安丽凤[1] 季顺欣[1] 夏祥河[1] Huang Jingwen;Han Xue;Zhao Xu;Li Xubo;Dong Xingzhong;An Lifeng;Ji Shunxin;Xia Xianghe(Jiamusi College,Heilongjiang University of Traditional Chinese Medicine,Jiamusi 150040,China)

机构地区：[1]黑龙江中医药大学佳木斯学院,150040

出　　处：《国际中医中药杂志》2019年第12期1353-1359,共7页International Journal of Traditional Chinese Medicine

基　　金：黑龙江省教育厅科学技术研究面上项目(12541765)。

摘　　要：目的运用网络药理学及生物信息学方法,预测三七治疗和改善瘢痕增生的分子机制,为其临床应用提供参考。方法通过中药系统药理学数据库分析平台获取三七的活性成分及作用靶点,Genecards数据库(https://www.genecards.org/)和OMIM数据库(https://omim.org/)搜集瘢痕的相关活性成分及作用靶点;运用Cytoscape 3.6.1软件构建"药物-化学成分-靶点-疾病"网络图;运用STRING数据库(https://string-db.org/)构建蛋白互作网络图;对关键靶点进行基因本体(gene ontology,GO)富集分析和京都基因与基因组百科全书数据库(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析。结果从三七中筛选出7个化学成分,包括β-谷甾醇、槲皮素、豆甾醇等;108个相关靶点,包括蛋白激酶(protein kinase B,AKT1)、c-Jun氨基端激酶、重组人有丝分裂原激活蛋白激酶(recombinant human mitogen-activated protein kinase 1,MAPK1)、IL-6等,主要通过磷脂酰肌醇3激酶(phosphatidylinositol 3 kinase,PI3K)/AKT信号通路、MAPK信号通路和TNF信号通路等多条信号通路发挥治疗和改善瘢痕的作用。结论本研究基于网络药理学的方法初步预测了三七治疗瘢痕的相关靶点及信号通路,可为后续研究提供参考。Objective We predicted the molecular mechanism of Panax notoginseng in the treatment and improvement of scar hyperplasia,by using the methods of network pharmacology and bioinformatics.Methods We collected of related active constituents and targets of Panax notoginseng by retrieving TCM systems pharmacology database and analysis platform(TCMSP),and collected of related active constituents and targets of scar by Genecards database and OMIM database.Cytoscape 3.6.1 software was used to construct"drugs-chemical components-targets-diseases"interaction network diagram.The protein in teraction network map(PPI)was constructed by STRING database.The key targets were used to analyze gene ontology(GO)enrichment and kyoto encyclopedia of genes and genome(KEGG)pathway enrichment.Results Totally 7 chemical compponents,including beta-sitosterol,quercetin,Stigmasterol and etc.and 108 targets,including AKT1,JUN,MAPK1,IL6 and ect.Panax notoginseng exerts its effects on scar mainly by acting on signal pathways,including PI3K-AKt signal pathway,MAPK signal pathway,TNF signal pathway and ect.Conclusions Based on the methodology of network pharmacology,this study preliminarily predicted the major targets and pathways Panax notoginseng in the treatment of scar,providing a direction for further studies.

关 键 词：三七 瘢痕 网络药理学 靶点 信号通路 

分 类 号：TP3[自动化与计算机技术—计算机科学与技术]