木犀草素通过抗氧化应激和抑制COX-2信号通路抑制哮喘幼鼠气道炎症  

Luteolin inhibits airway inflammation in asthmatic young mice via antioxidant stress and COX-2 signaling pathway

作　　者：邹华 王颖 秦晓宇 张宝辉[2] ZOU Hua;WANG Ying;QIN Xiao-yu;ZHANG Bao-hui(Department of Pediatrics,Yingkou Central Hospital,Yingkou 115003;Deptment of Neurobiology,Basic Medical College,China Medical University,Shenyang 110122,China)

机构地区：[1]辽宁省营口市中心医院儿科,辽宁营口115003 [2]中国医科大学神经生物学教研室,辽宁沈阳110122

出　　处：《解剖科学进展》2019年第6期634-636,共3页Progress of Anatomical Sciences

基　　金：国家自然科学基金(81070011)

摘　　要：目的探讨木犀草素对哮喘幼鼠的防治作用及可能机制。方法36只BALB/c幼鼠,雌雄不限,随机分为对照组、哮喘组和木犀草素组,卵蛋白致敏的方法制备哮喘幼鼠模型。最后一次激发后24h,处死大鼠后取肺组织,HE染色观察各组幼鼠肺组织病理学改变;取大鼠血清检测SOD、MDA、GSH-Px及T-AOC变化;采用实时PCR和Western-blot法检测各组幼鼠肺组织COX-2、PGE2、TNF-α及IL-1βmRNA和蛋白表达。结果与对照组相比,哮喘组幼鼠气道上皮组织结构紊乱,肺内可见大量炎性细胞浸润,木犀草素明显减轻哮喘幼鼠肺内炎性浸润,降低哮喘幼鼠血清MDA含量,上调SOD、GSH-Px、T-AOC水平,下调哮喘幼鼠肺组织COX-2、PGE2、TNF-α及IL-1β的mRNA和蛋白表达(P<0.01)。结论木犀草素减轻哮喘幼鼠气道炎症,与其抗氧化应激和抑制COX-2信号通路相关。Objective To investigate the therapeutic effect and possible mechanism of luteolin on the asthmatic young mice.Methods 36 BALB/c mice,male and female,were randomly divided into control group,asthma group(established by ovalbumin)and luteolin group.24 hours after the last stimulation,the lung tissues of the mice were sacrificed,and the pathological changes of lung tissues were observed by HE staining.The contents of SOD,MDA,GSH-Px and T-AOC in serum were detected.The expressions of COX-2,PGE2,TNF-αand IL-1βwere determined by real-time PCR and Western-blot.Results Compared with the control group,a large number of inflammatory infiltration cells and disordered epithelium in the lung tissues were found in the asthmatic group.Luteolin alleviated the inflammatory infiltration,down-regulated MDA content in serum and the expression levels of COX-2,PGE2,TNF-αand IL-1βin lung tissue,upregulated the levels of SOD,GSH-Px and T-AOC in lung tissue of asthmatic young mice(P<0.01).Conclusion Luteolin inhibited the airway inflammation in asthmatic young mice,which is related to antioxidant stress and inhibition of COX-2 signaling pathway.

关 键 词：哮喘 木犀草素 氧化应激 COX-2 幼鼠 

分 类 号：R562.25[医药卫生—呼吸系统]