MTA1基因调节神经胶质母细胞瘤细胞U-87-MG侵袭与迁移  

MTA1 gene regulates the invasion and migration of glioblastoma cell line U-87-MG

作　　者：朴成浩[1] 王效杰[2] 谭力力[3] 贺丽[4] 王一维[2] PIAO Cheng-hao;WANG Xiao-jie;TAN Li-li;HE Li;WANG Yi-wei(Radiology Department of Second Affiliated Hospital,Shenyang Medical College,Shenyang 110035;Department of Human Anatomy,Shenyang Medical College,Shenyang 110034;Department of Cardiac Rehabilitation,Second Affiliated Hospital,Shenyang Medical College,Shenyang 110035;Gastroenterology Department of Second Affiliated Hospital,Shenyang Medical College,Shenyang 110035)

机构地区：[1]沈阳医学院附属第二医院放射线科,沈阳110035 [2]沈阳医学院人体解剖教研室,沈阳110034 [3]沈阳医学院附属第二医院心脏康复科,沈阳110035 [4]沈阳医学院附属第二医院消化内科,沈阳110035

出　　处：《解剖科学进展》2019年第6期670-672,676,共4页Progress of Anatomical Sciences

基　　金：辽宁省自然科学基金计划重点项目(20170520040);辽宁省重点研发计划指导计划项目(2017225061)

摘　　要：目的评价沉默MTA1基因对神经胶质瘤细胞U-87-MG侵袭与迁移能力的影响。方法应用慢病毒转染技术沉默MTA1基因的表达,通过Western blot实验与荧光共聚焦实验检测MTA1蛋白与F-actin蛋白的表达;通过Transwell实验评估细胞侵袭与迁移能力;Western blot实验检测E-cadherin,N-cadherin蛋白的表达。结果沉默MTA1基因可下调F-actin蛋白的表达水平,抑制U-87-MG细胞的侵袭与迁移能力,并且上调N-cadherin蛋白,下调E-cadherin蛋白的表达。结论沉默MTA1基因抑制胶质母细胞瘤细胞系U-87-MG的侵袭与迁移能力与影响F-actin的集聚与EMT相关蛋白N-cadherin与E-cadherin表达相关。Objective To evaluate the effect of silencing MTA1 on the invasion and migration of glioblastoma cell line U-87-MG. Methods The expression of MTA1 was silenced by lentiviral transfection technique. The expression of MTA1 protein and F-actin protein was detected by Western blot and fluorescence confocal assay. The invasion and migration abilities of U-87-MG cells were evaluated by Transwell assay. The expression of E-cadherin and N-cadherin protein was detected by Western blot. Results Silencing MTA1 down-regulated the expression level of F-actin protein, inhibited the invasion and migration of U-87-MG cells, and up-regulated the expression levels of N-cadherin protein, and downregulated the expression levels of E-cadherin protein. Conclusion Silencing MTA1 inhibits the invasion and migration of glioblastoma cell line U-87-MG by affecting the accumulation of F-actin and the expression of EMT-related proteins Ncadherin and E-cadherin.

关 键 词：神经胶质母细胞瘤 MTA1 侵袭 迁移 

分 类 号：R739.41[医药卫生—肿瘤]