袖状胃切除术对2型糖尿病大鼠肾氧化应激的影响  

Effect of sleeve gastrectomy on renal oxidative stress in type 2 diabetic rats

作　　者：张浩[1] 杨可钰 王勇[1] Zhang Hao;Yang Keyu;Wang Yong(Department of General Surgery,Fourth Affiliated Hospital of China Medical University,Shenyang 110032,China)

机构地区：[1]中国医科大学附属第四医院普外科,沈阳110032

出　　处：《国际外科学杂志》2019年第12期819-824,共6页International Journal of Surgery

基　　金：辽宁省自然科学基金(2016010864-01)。

摘　　要：目的探讨袖状胃切除术后2型糖尿病大鼠肾氧化应激的变化及其对肾功能的影响。方法20只雄性SD大鼠采用高脂饮食喂养同时腹腔注射链脲佐菌素的方法诱导2型糖尿病模型。成功诱导模型17只,采用随机数字表法分为假手术组(n=7)和袖状胃切除术(SG)组(n=10),分别给予假手术和SG处理。测量术前、术后1、2、3、4周大鼠体重、24 h摄食量、空腹血糖和胰高血糖素样肽-1(GLP-1),于术后第4周检测肾组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活力和丙二醛含量以及血尿素氮、肌酐含量和24 h尿微量白蛋白(UALB)含量。符合正态分布的计量资料以均数±标准差(Mean±SD)表示,两组间比较采用t检验。结果SG组术后1~4周空腹血糖均低于假手术组[(11.13±3.27)mmol/L比(16.74±4.10)mmol/L、(9.53±2.82)mmol/L比(19.31±3.66)mmol/L、(6.69±2.44)比(20.84±2.71)mmol/L、(6.58±2.96)mmol/L比(19.99±2.85)mmol/L,P均<0.05]。术后1~4周SG组血清GLP-1水平均高于假手术组[(22.61±2.92)pg/mL比(15.42±2.39)pg/mL、(24.72±3.02)pg/mL比(16.20±2.26)pg/mL、(24.59±2.85)pg/mL比(15.84±2.75)pg/mL、26.15±3.23)pg/mL比(15.77±2.79)pg/mL,P均<0.05]。术后4周,SG组血尿素氮(5.34±0.82)mmol/L、肌酐(39.78±6.13)μmol/L、UALB(31.18±6.88)mg/24 h和肾脏丙二醛(5.84±0.85)nmol/mg低于假手术组[血尿素氮(9.08±1.54)mmol/L、肌酐(64.07±8.63)μmol/L、UALB(67.64±9.07)mg/24 h、丙二醛(10.78±1.28)nmol/mg],差异均有统计学意义(P均<0.05)。术后4周,肾脏SOD(620.05±55.98)U/mg、CAT(24.72±2.28)U/mg、GSH-Px(281.53±27.99)U高于假手术组[SOD(392.52±45.97)U/mg、CAT(15.62±2.46)U/mg、GSH-Px(164.71±21.83)U],差异均有统计学意义(P均<0.05)。结论SG可能通过降低肾脏氧化应激水平来改善肾脏功能。Objective To investigate the changes of oxidative stress in kidney of type 2 diabetic rats after sleeve gastrectomy and its effect on renal function.Methods Twenty male SD rats were fed with high-fat diet and injected with streptozotocin intraperitoneally to induce type 2 diabetes mellitus.Seventeen successful induction models were divided into sham operation group(n=7)and sleeve gastrectomy(SG)group(n=10)by random number table method,and were treated with sham operation and SG respectively.The body weight,24-hour food intake,fasting blood glucose(FBG)and serum glucagon-like peptide-1(GLP-1)were measured before and 1,2,3 and 4 weeks after operation.The activities of superoxide dismutase(SOD),catalase,glutathione peroxidase(GSH-Px)and malondialdehyde in renal tissue as well as blood urea nitrogen(BUN),creatinine levels and 24-hour urinary microalbumin(UALB)were measured 4 weeks after operation.Masurement data conforming to normal distribution were expressed as Mean±SD,and t-test was used for comparison between two groups.Results At the 1st,2nd,3rd,4th weeks after operation,the levels of FBG in SG group was lower than those in sham operation group[(11.13±3.27)mmol/L vs(16.74±4.10)mmol/L,(9.53±2.82)mmol/L vs(19.31±3.66)mmol/L,(6.69±2.44)mmol/L vs(20.84±2.71)mmol/L,(6.58±2.96)mmol/L vs(19.99±2.85)mmol/L,all P<0.05].In the same period,the levels of serum GLP-1 in SG group was higher than those in sham operation group[(22.61±2.92)pg/mL vs(15.42±2.39)pg/mL,(24.72±3.02)pg/mL vs(16.20±2.26)pg/mL,(24.59±2.85)pg/mL vs(15.84±2.75)pg/mL,(26.15±3.23)pg/mL vs(15.77±2.79)pg/mL,all P<0.05].At the 4th week after operation,BUN[(5.34±0.82)mmol/L],creatinine[(39.78±6.13)]μmol/L,UALB[(31.18±6.88)mg/24 h]and renal creatinine[(5.84±0.85)nmol/mg]in SG group were lower than those in sham operation group[BUN(9.08±1.54)mmol/L,creatinine(64.07±8.63)μmol/L,UALB(67.64±9.07)mg/24 h,creatinine(10.78±1.28)nmol/mg],and the differences were statistically significant(all P<0.05).Renal SOD[(620.05±55.98)U/mg],CAT[(24.72±2

关 键 词：糖尿病 2型 糖尿病肾病 超氧化物类 袖状胃切除术 氧化应激 

分 类 号：R58[医药卫生—内分泌]