Silicon impacts on soil microflora under Ralstonia Solanacearum inoculation  

作　　者：LIN Wei-peng JIANG Ni-hao PENG Li FAN Xue-ying GAO Yang WANG Guo-ping CAI Kun-zheng 

机构地区：[1]College of Natural Resources and Environment,South China Agricultural University,Guangzhou 510642,P.R.China [2]Tea Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,P.R.China [3]Key Laboratory of Tropical Agricultural Environment in South China,Ministry of Agriculture,Guangzhou 510642,P.R.China [4]College of Agriculture,South China Agricultural University,Guangzhou 510642,P.R.China [5]College of Horticulture,South China Agricultural University,Guangzhou 510642,P.R.China

出　　处：《Journal of Integrative Agriculture》2020年第1期251-264,共14页农业科学学报（英文版）

基　　金：financially supported by grants from the National Natural Science Foundation of China (31370456);the Doctoral Foundation of the Ministry of Education of China (20124404110007);the Natural Science Foundation of Guangdong Province of China (S2012010010331 and 2017A030313177);the Project of International, as well as Hong Kong, Macao & Taiwan Science and Technology Cooperation Innovation Platform in Universities in Guangdong Province, China (2014KGJHZ004)

摘　　要：Silicon(Si) can increase plant resistance against bacterial wilt caused by Ralstonia solanacearum and enhance plant immune response. However, whether Si alleviates soil-borne disease stress through altering soil microbial community component and diversity is not clear. In this study, effects of Si application under R. solanacearum inoculation with or without plant on soil bacterial and fungal communities were investigated through high-throughput pyrosequencing technique. The results showed that Si addition significantly reduced bacterial wilt incidence. However, Si did not reduce the amount of R. solanacearum in rhizosphere soil. Principal components analysis showed that soil microbial community composition was strongly influenced by Si addition. Total 63.7% bacterial operational taxonomic units(OTUs) and 43.8% fungal OTUs were regulated by Si addition regardless of the presence of tomato plants, indicating the independent effects of Si on soil microbial community. Si-added soil harbored a lower abundance of Fusarium, Pseudomonas, and Faecalibacterium. Our finding further demonstrated that exogenous Si could significantly influence soil microbial community component, and this may provide additional insight into the mechanism of Si-enhanced plant resistance against soil-borne pathogens.Silicon(Si) can increase plant resistance against bacterial wilt caused by Ralstonia solanacearum and enhance plant immune response. However, whether Si alleviates soil-borne disease stress through altering soil microbial community component and diversity is not clear. In this study, effects of Si application under R. solanacearum inoculation with or without plant on soil bacterial and fungal communities were investigated through high-throughput pyrosequencing technique. The results showed that Si addition significantly reduced bacterial wilt incidence. However, Si did not reduce the amount of R. solanacearum in rhizosphere soil. Principal components analysis showed that soil microbial community composition was strongly influenced by Si addition. Total 63.7% bacterial operational taxonomic units(OTUs) and 43.8% fungal OTUs were regulated by Si addition regardless of the presence of tomato plants, indicating the independent effects of Si on soil microbial community. Si-added soil harbored a lower abundance of Fusarium, Pseudomonas, and Faecalibacterium. Our finding further demonstrated that exogenous Si could significantly influence soil microbial community component, and this may provide additional insight into the mechanism of Si-enhanced plant resistance against soil-borne pathogens.

关 键 词：bacterial wilt deep pyrosequencing Ralstonia solanacearum SILICON soil microbial community 

分 类 号：S432.4[农业科学—植物病理学] S154.3[农业科学—农业昆虫与害虫防治]