樟树胚性愈伤组织诱导与体胚发生研究  

Embryogenic Callus Induction and Somatic Embryogenesis from Immature Zygotic Embryos of Cinnamomum camphor

作　　者：戴小英[1] 刘新亮 章挺[1] 李江[1] 周诚[1] DAI Xiao-ying;LIU Xin-liang;ZHANG Ting;LI Jiang;ZHOU Cheng(Camphor Engineering and Technology Research Centre for National Forestry and Grassland Administration,Jiangxi Academy of Forestry,Nanchang 330032,China)

机构地区：[1]江西省林业科学院国家林业和草原局樟树工程技术研究中心

出　　处：《江西农业大学学报》2019年第6期1120-1129,共10页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家“十三·五”重点研发计划项目（2016YFD0600605）;国家“十三·五”重点研发计划子课题（2017YFD0601102）;江西省林科院博士启动项目（2017522701）~~

摘　　要：以樟树未成熟合子胚为外植体,探讨了外植体取材时间、植物生长调节剂配比、附加有机物等因素对胚性愈伤组织诱导、增殖培养及体细胞胚胎发生的影响。结果表明:不同采集时期的合子胚愈伤组织诱导率差异显著,只有心形胚时期的合子胚才能诱导胚性愈伤组织;在改良MS+4.0 mg/L 6-BA+0.5 mg/L NAA培养基初诱导情况下,后接入改良MS+1.0 mg/L 6-BA+0.2 mg/L 2,4-D培养基中,胚性愈伤组织诱导率可达77.78%;外加添加200 mg/L水解络蛋白和200 mg/L环糊精可保持胚性愈伤组织增殖和体细胞胚诱导,胚性愈伤组织增殖系数达3.86,每克愈伤组织平均分化116.67个体细胞胚,胚性愈伤组织继代周期以25~30 d为宜。在改良MS+200 mg/L环糊精+1.0 mg/L ABA+0.5 mg/L IAA中可以促使体胚成熟萌发,萌发率可达52.78%。将萌发的芽体转接到MS+0.1 mg/L BA+0.5 mg/L IAA+0.2 g/L AC生根培养可获得完整植株。Taking immature zygotic embryos of camphor tree as explants,the effects of explant harvesting time,plant growth regulator ratio and additional organic substances on embryogenic callus induction,proliferation culture and somatic embryogenesis were studied. The results showed that the induction rate of zygotic embryo callus was different at different collection stages and only zygotic embryos in heart-shaped embryo stage could induce embryogenic callus.The induction rate of embryogenic callus was 77.78% when the improved MS+4.0 mg/L 6-BA+0.5 mg/L NAA medium was first induced and then inserted into the improved MS+1.0 mg/L 6-BA+0.2 mg/L 2,4-D medium.Adding 200 mg/L hydrolyzed complex protein and 200 mg/L cyclodextrin could maintain the proliferation of the embryogenic callus and somatic embryo induction.In this conditions,the proliferation coefficient of embryogenic callus was 3.86 and the average number of somatic embryos was 116.67 per gram.The appropriate subculture cycle of embryogenic callus was about 25 to 30 days.The improved MS+200 mg/L cyclodextrin+1.0 mg/L ABA+0.5 mg/L IAA medium could promote somatic embryo maturation and germination,and the germination rate could reach 52.78%.Complete plants could be obtained by transferring the germinated buds to MS + 0.1 mg/L BA + 0.5 mg/L IAA + 0.2 g/L rooting medium.

关 键 词：樟树 胚性愈伤组织 体细胞胚胎发生 诱导 增殖 

分 类 号：S792.23[农业科学—林木遗传育种]