多光束超分辨显微成像系统的设计与光束重合度优化  

Design of Multi-beam Super-Resolution Microscopic Imaging System and Optimization of Beam Coincidence Degree

作　　者：张力 刘铁诚[3] 陈旭 余彬彬 张金仓 王中阳[2] ZHANG Li;LIU Tiecheng;CHEN Xu;YU Binbin;ZHANG Jinchang;WANG Zhongyang(Department of Physics,University of Shanghai,Shanghai 200444,China;Shanghai Advanced Research Institute,Chinese Academy of Sciences,Shanghai 201210,China;Shanghai Institute of Optics and Fine Mechanics,Chinese Academy of Sciences,Shanghai 201800,China;School of Optical-electrical and Computer Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)

机构地区：[1]上海大学物理系,上海200444 [2]中国科学院上海高等研究院,上海201210 [3]中国科学院上海光学精密机械研究所,上海201800 [4]上海理工大学光电信息与计算机工程学院,上海200093

出　　处：《上海理工大学学报》2019年第6期563-569,共7页Journal of University of Shanghai For Science and Technology

基　　金：国家科技部项目（16YFC0100603）;上海市科委科研计划项目（18DZ1100403）

摘　　要：为了提升超分辨显微成像系统的分辨能力,自主设计并搭建了一套多光束超分辨显微成像系统。该成像系统包括有3束激光束,分别为405 nm实心激发光束、488 nm实心激发光束以及488 nm空心损耗光束。通过Labview程序控制纳米位移台与单光子测器间的数据采集,采用80 nm金纳米颗粒探针扫描方式对该成像系统进行纳米尺度的光束调制,完成了多光束显微成像系统的光学调节,主要包括有多光束的光斑整形、多光束间的光束空间重合度优化以及对应光斑强度分布的仿真模拟。通过已知绿色荧光蛋白样品的测试结果,说明了该方法对于系统中光束空间重合度的提升作用与效果。In order to improve the resolution of the super-resolution microscopic imaging system,a multi-beam super-resolution microscopic imaging system was designed and set up.The imaging system includes three laser beams:405 nm excitation solid beam,488 nm excitation solid beam,and 488 nm annular beam.Labview was used to control the data collection between the nano-stage and the single photon detector and 80 nm gold nanoparticle probe was used to modulate the laser beam on nanoscale.It mainly includes the light beam shaping of multi-beam,the optimization of the coincidence degree,and simulation of intensity distribution.From the test results of the green fluorescent protein sample,it illustrates by using this method,the spatial coincidence degree of beams can be improved in the system.

关 键 词：多光束超分辨 金纳米颗粒 光斑整形 重合度优化 

分 类 号：O438[机械工程—光学工程]