FKS家族基因对酿酒酵母压力耐性的影响  

Effects of FKS Family Genes on the Ability of Saccharomyces cerevisiae Stress Resistance

作　　者：杨歌 王金晶[1,2] 李佳 郑飞云[1,2] 刘春凤 李永仙[1,2] 钮成拓 李崎[1,2] YANG Ge;WANG Jinjing;LI Jia;ZHENG Feiyun;LIU Chunfeng;LI Yongxian;NIU Chengtuo;LI Qi(Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214122,China;Lab of Brewing Science and Engineering,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122 [2]江南大学酿酒科学与工程研究室,江苏无锡214122

出　　处：《食品与生物技术学报》2019年第10期126-134,共9页Journal of Food Science and Biotechnology

基　　金：国家863计划项目(2013AA102106-03);国家自然科学基金项目(31271919,31571942,31301539);江苏省自然科学基金项目(BK20150159);江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：FKS作为1,3-β-葡聚糖合成酶基因家族,对维持酿酒酵母细胞壁有重要作用。为探究FKS家族基因对酿酒酵母细胞抗逆性及其合成乙醇的影响,通过同源重组的方法分别构建了FKS1、FKS2和FKS3基因的缺失菌株,并比较重组菌株和原始菌株的性能差异。结果表明,FKS1缺陷菌株细胞壁的1,3-β-葡聚糖含量低于原始菌株60%,生长性能、抗胁迫性以及合成乙醇能力都较差。FKS3缺陷菌株的生长性能和胁迫性能与原菌株相似,在发酵环境中抵抗外界环境能力和合成乙醇能力优于原始菌株。因此,FKS1基因对维持酵母细胞活性和抗逆有重要作用,而FKS3基因对抗逆有负面作用。The FKS family genes which encoded 1,3-β-glucan synthase are important to maintain the integrity of Saccharomyces cerevisiae cell wall.To illustrate the functions of FKS family genes on the ability of S.cerevisiae to tolerate the environmental stresses and ethanol production,the FKS1,FKS2,and FKS3 knocked out strains were constructed by homologous recombination,then the physiological properties of wile-type strain and mutant strains were measured and compared.The results showed that the content of 1,3-β-glucan in cell wall of the FKS1 knocked out strain was 60%lower compared to the wild type.The knockout of FKS1 gene also weakened the abilities of growth,stress resistance and ethanol production for S.cerevisiae.Furthermore,the growth and stress resistance abilities of FKS3 gene knocked out strain were similar to those of the wild-type strain,however its abilities to tolerate the fermentation environment and to produce ethanol were better than those of the wild-type strain.Therefore,the FKS1 gene was important for S.cerevisiae to keep the cells activity and to resist the environmental stresses while the FKS3 gene negatively influenced the ability of S.cerevisiae to resist outer stresses.

关 键 词：细胞壁 1 3-β-葡聚糖 压力耐受 乙醇合成 

分 类 号：Q815[生物学—生物工程]