RAL-STAINER自动染色仪对抗酸染色的性能评估  

Evaluation of performance of gram staining for various clinical microbiological specimens using RAL-STAINER automatic staining instrument

作　　者：徐阳颺 李志夫[1] 赵越薇 李富升 娄加陶[1] XU Yangyang;LI Zhifu;ZHAO Yuewei;LI Fusheng;LOU Jiatao(Department of Clinical Laboratory,Shanghai Chest Hospital Affiliated to Shanghai Jiaotong University,Shanghai 200030,China)

机构地区：[1]上海交通大学附属胸科医院检验科

出　　处：《国际检验医学杂志》2020年第1期53-55,共3页International Journal of Laboratory Medicine

基　　金：上海市胸科医院多学科协同临床研究创新项目（YJXT20190201）

摘　　要：目的 评估RAL-STAINER自动染色仪对抗酸染色的性能。方法 收集标本,分别进行手工抗酸染色和仪器抗酸染色,评价自动染色仪的染色效果的合格率与手工染色的染色结果的一致率、仪器染色的重复性、与室间质评预期结果的符合率、交叉污染和工作性能等。结果 RAL-STAINER自动染色仪对各类标本抗酸染色背景基本无杂质,细菌和细胞色泽鲜明、结构清楚,染色效果的合格率为100%。自动染色仪与手工抗酸染色一致率为100%。仪器染色的重复性为100%。使用自动染色仪,两次室间质评结果均100%符合预期。自动染色仪批量染色时,无交叉污染。自动染色仪操作简便、通量高、染色效果稳定、生物安全性好。结论RAL-STAINER自动染色仪对抗酸染色的性能较好。Objective To evaluate the performance of RAL-STAINER automatic colorimeter in gram staining.Methods The samples were collected for manual and instrumental gram staining to evaluate the qualification rate of automatic staining instrument,the consistency rate with the results of manual staining,the repeatability of instrumental staining,the consistency rate with the expected results of external quality assessment,cross contamination and working performance.Results The results showed that RAL-STAINER had no impurity in the background of gram staining,bacteria and cells had bright color and clear structure,and the qualified rate of staining effect was 100%.The consistency of automatic staining instrument and manual gram staining instrument was 100%.The repeatability of staining was 100%.Using the automatic staining instrument,the results of the two external quality assessments were 100%in line with the expectation.There was no cross contamination in batch staining with automatic colorimeter.The automatic staining instrument has the advantages of simple operation,high flux,stable staining effect and good biological safety.Conclusion The performance of RAL-STAINER is relatively good for gram staining.

关 键 词：RAL-STAINER 微生物 染色仪 抗酸染色 性能评估 

分 类 号：R446.5[医药卫生—诊断学]