ELISA在东莞地区无偿献血中检测人类嗜T淋巴细胞病毒抗体的应用  

Application of ELISA in the detection of human T-lymphocyte virus antibody detected by in blood donors in Dongguan

作　　者：王庆[1] 余霖[1] 陈庆恺[1] 邹姣丽[1] 何子毅[1] 陈少彬[1] WANG Qing;YU Lin;CHEN Qingkai;ZOU Jiaoli;HE Ziyi;CHEN Shaobin(Departmant of Laboratory,Dongguan Blood Center,Guandong Province,Dongguan 523930,China)

机构地区：[1]广东省东莞市中心血站检验科

出　　处：《中国医药导报》2019年第34期157-160,共4页China Medical Herald

基　　金：广东省东莞市社会科技发展(一般)项目(201750715026369)

摘　　要：目的探讨酶联免疫吸附试验(ELISA)在广东省东莞地区无偿献血者中检测人类嗜T淋巴细胞病毒(HTLV)抗体的应用,为开展相应检测和保障血液安全提供参考依据。方法选取2016年3月~2017年1月在广东省东莞地区参加无偿献血而留取的血浆标本68323份,采用ELISA作为HTLV抗体的筛查方法,对筛查阳性者采用电化学发光免疫分析法(ECLIA)进行补充检测并采用蛋白免疫印迹试验(WB)进行确证试验。结果经ELISA筛查HTLV抗体阳性标本26份,筛查阳性率为0.038%;26份标本经ECLIA检测呈阳性标本9份;经WB确证阳性2份,确证阳性率为0.003%,该2例均为福建籍;HTLV抗体的阳性预期值为7.69%(2/26);不同性别、年龄、学历、是否首次献血和是否为东莞户籍的献血者筛查阳性率差异无统计学意义(P>0.05)。结论东莞地区无偿献血者HTLV抗体的筛查和确证阳性率均较低,且阳性预期值也不高,建议采用特异性更强的ELISA试剂或采用ECLIA进行筛查;招募时可适当加强对福建籍献血者的征询。Objective To investigate the application of enzyme-linked immunosorbent assay(ELISA)in the detection of human T-lymphocyte virus(HTLV)antibody in blood donors in Dongguan,and to carry out the corresponding test and ensure the blood safety to provide a reference basis.Methods A total of 68323 plasma samples were collected from March 2016 to January 2017 in the blood donation in Dongguan Guangdong Province.ELISA was used as a screening method for human T-lymphocyte virus antibodies.Positive samples in the screening were supplemented by electrochemiluminescence(ECLIA)and confirmed by Western blotting(WB).Results Twenty-six samples of HTLV antibody-positive samples were screened by ELISA,the positive rate of screening was 0.038%.Nine samples were positive by ECLIA in the 26 samples,2 samples were positive by WB,and the positive rate was 0.003%,two cases were all from Fujian Province.The positive predictive value of HTLV antibody was 7.69%(2/26).There were no statistically significant differences in the positive screening rates among blood donors of different genders,ages,educational backgrounds,whether they had donated blood for the first time and whether they were registered in Dongguan(P>0.05).Conclusion The positive rate of screening and confirmation of HTLV antibodies in blood donors in Dongguan is low,and the positive predictive value is not high.It is recommended to use more specific ELISA or ECLIA for screening.When recruiting,the recruitment of Fujian blood donors can be appropriated strengthened.

关 键 词：无偿献血者 人类嗜T淋巴细胞病毒 酶联免疫吸附试验 蛋白免疫印迹试验 

分 类 号：R446.6[医药卫生—诊断学]