EPO在缺氧环境下对神经保护机制的研究  

Research on neuroprotective mechanism of EPO in hypoxic environment

作　　者：兰海霞 春英 呼格吉乐[2] LAN Haixia;CHUN Ying;HU Gejile(Department of Paediatrics,the 969th Hospital of the People′s Liberation Army,Inner Mongolia Autonomous Region,Hohhot010051,China;Department of Clinical Laboratory,the Affiliated Hospital of Inner Mongolia Medical University,Inner Mongolia Autonomous Region,Hohhot010059,China)

机构地区：[1]解放军第九六九医院儿科,内蒙古呼和浩特010051 [2]内蒙古医科大学附属医院检验科,内蒙古呼和浩特010059

出　　处：《中国医药导报》2020年第1期9-12,共4页China Medical Herald

基　　金：内蒙古自治区自然科学基金项目（2018LH08047）

摘　　要：目的讨论促红细胞生成素(EPO)对氯化钴(CoCl2)缺氧环境下U251细胞增殖以及对BMI1基因转录水平的影响,为研究EPO对神经保护的作用奠定基础。方法将实验细胞分为对照组(0μmol/mL CoCl2)、CoCl2组(400μmol/mL CoCl2)和CoCl2+EPO组(400μmol/mL CoCl2和75 U/mL EPO)。通过CCK-8法检测CoCl2对细胞增殖的影响来评价缺氧模型;CCK-8法检测缺氧条件下EPO对U251细胞增殖的影响;qPCR法检测CoCl2组和CoCl2+EPO组中BMI1基因转录水平的变化。结果 CoCl2组48 h细胞增殖水平低于对照组比较,差异有统计学意义(P <0.05);CoCl2+EPO组增殖水平高于CoCl2组胞,差异有统计学意义(P <0.05)。CoCl2+EPO组BMI1基因转录水平高于CoCl2组,差异有统计学意义(P <0.05)。结论本研究成功构建CoCl2对人神经胶质U251细胞的缺氧模型,EPO在缺氧环境下通过上调BMI1基因的表达促进U251细胞的增殖,EPO对神经细胞具有保护作用。Objective To discuss the effect of erythropoietin(EPO) on the proliferation of U251 cells and the levels of BMI1 gene transcription in hypoxia environment of cobalt chloride(CoCl2), and lay the foundation for studying the role of EPO on neuroprotection. Methods The experimental cells were divided into control group(0 μmol/mL CoCl2), CoCl2 group(400 μmol/mL CoCl2) and CoCl2+EPO group(400 μmol/mL CoCl2 and 75 U/mL EPO). The hypoxia model was evaluated by measuring the effect of CoCl2 on cell proliferation by CCK-8 method;the effect of EPO on the proliferation of U251 cells was detected by CCK-8 method;the changes of BMI1 gene transcription levels in CoCl2 group and CoCl2+EPO group was detected by qPCR method. Results The cell proliferation levels in the CoCl2 group at 48 h were lower than those in the control group, and the differences were statistically significant(P < 0.05);the proliferation levels in the CoCl2+EPO group were higher than those in the CoCl2 group, with a statistically significant differences(P <0.05). The levels of transcription of BMI1 gene in CoCl2+EPO group were higher than those in CoCl2 group, and the differences were statistically significant(P < 0.05). Conclusion This study successfully constructed a hypoxia model of human glial U251 cells with CoCl2. EPO promotes U251 cell proliferation by up-regulating the expression of the BMI1 gene under hypoxic conditions, and EPO has a protective effect on nerve cells.

关 键 词：促红细胞生成素 缺氧 神经保护 BMI1基因 

分 类 号：R692.5[医药卫生—泌尿科学]