SPE-UPLC法同时测定复方荆防颗粒中的升麻素苷和5-O-甲基维斯阿米醇苷的含量  

Simultaneous determination of Prim-O-Glucnylcimifugin and 4′-O-β-glucosyl-5-O-Methylvismmiside in Compound Jingfang Granules by SPE-UPLC

作　　者：江丽 余辉 曹月霞 龚春燕 JIANG Li;YU Hui;CAO Yuexia;GONG Chunyan(Department of Pharmacy,Institute of Dermatology,Chinese Academy of Medical Sciences and Peking Union College,Jiangsu Province,Nanjing210042,China)

机构地区：[1]中国医学科学院北京协和医学院皮肤病医院药剂科

出　　处：《中国医药导报》2020年第1期33-37,共5页China Medical Herald

基　　金：中国医学科学院医学与健康科技创新工程资助项目（2017-I2M-1-011）

摘　　要：目的建立固相萃取-超高效液相色谱(SPE-UPLC)法同时测定复方荆防颗粒中升麻素苷和5-O-甲基维斯阿米醇苷的含量。方法采用固相萃取系统对复方荆防颗粒进行处理,色谱柱为ACQUITY UPLC HSS T3(2.1 mm×100 mm,1.8μm),流动相为乙腈-水,梯度洗脱,流速为0.4 mL/min,检测波长254 nm,柱温为35℃。结果升麻素苷与5-O-甲基维斯阿米醇苷在5~80 mg/L范围内均有良好线性关系,线性回归方程分别为A=40 195C-248.67(r=1.0000),A=42 577C+12 782(r=1.0000);平均加样回收率为98.11%、99.28%,RSD为1.92%、1.90%(n=6)。结论 SPE-UPLC法简便准确且重现性好,可有效控制复方荆防颗粒的质量。Objective To establish a solid phase extraction and ultra performance liquid chromatography(SPE-UPLC method for the simultaneous determination of Prim-O-Glucnylcimifugin and 4′-O-β-glucosyl-5-O-Methylvismmiside in Compound Jingfang Granules. Methods Samples were processed by the solid phase extraction system, and the separation was performed on ACQUITY UPLC HSS T3(2.1 mm×100 mm,1.8 μm) with acetonitrile-water mobile phase for the gradient elution. The flow rate was 0.4 mL/min, the detection wavelength was 254 nm, and the column temperature was 35℃. Results The calibration curve for Prim-O-Glucnylcimifugin and 4′-O-β-glucosyl-5-O-Methylvismmiside in the range of 5-80 mg/L showed good liner. The linear regression equation for Prim-O-Glucnylcimifugin were A = 40 195 C-248.67(r =1.0000) and the linear regression equation for 4′-O-β-glucosyl-5-O-Methylvismmiside were A = 42 577 C+12 782(r =1.0000). The average recovery of Prim-O-Glucnylcimifugin and 4′-O-β-glucosyl-5-O-Methylvismmiside were 98.11% and 99.28%, and RSD were 1.92% and 1.90%(n = 6), respectively. Conclusion SPE-UPLC method is simple, accurate and reproducible and effectivly control the quality of Compound Jingfang Granules.

关 键 词：复方荆防颗粒 升麻素苷 5-O-甲基维斯阿米醇苷 固相萃取-超高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]