龙胆泻肝汤对阴道加德纳菌的体外抑制作用  

Inhibitory effects of Longdan Xiegan Decoction on Gardnerella vaginalis in vitro

作　　者：吴汉 敖梅英[1] 陈雪丽 陈来[1] 左爱仁[1] 谢燕飞[1] 邓可众[1] WU Han;AO Mei-ying;CHEN Xue-li;CHEN Lai;ZUO Ai-ren;XIE Yan-fei;DENG Ke-zong(Center Office of Traslational Medicine,College of Traditional Chinese Medicine,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China)

机构地区：[1]江西中医药大学中医学院转化医学中心

出　　处：《中成药》2020年第1期75-80,共6页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金地区项目(81260242);江西省自然科学基金青年项目(20142BAB214009);江西省教育厅科技计划青年项目(GJJ12540);江西省卫生厅中医药类项目(2013A083);江西省卫生计生委科技计划项目(20185526);江西中医药大学校级研究生创新专项资金项目(JZYC19S34)

摘　　要：目的研究龙胆泻肝汤对阴道加德纳菌(GV)的体外抑制作用。方法利用稀释法检测龙胆泻肝汤对GV增殖的影响,并测定药物的最低抑菌浓度,通过革兰氏染色法观察龙胆泻肝汤对GV黏附人宫颈癌Hela细胞的影响,MTT法检测龙胆泻肝汤对GV细胞毒性的影响,96孔微量板法检测龙胆泻肝汤对GV生物膜形成的影响,qRT-PCR法检测龙胆泻肝汤对GV BAP、sialidase mRNA表达的影响。结果龙胆泻肝汤水提物、70%醇提物和90%醇提物对GV的最低抑菌浓度分别为(62.5±3.6)、(15.6±1.5)、(125.0±2.8) mg/mL。龙胆泻肝汤70%醇提物(1.56、15.6 mg/mL)能降低GV对Hela细胞的黏附,抑制GV生物膜的形成(P<0.01),下调BAP、sialidase mRNA表达(P<0.05,P<0.01),龙胆泻肝汤70%醇提物(15.6 mg/mL)抑制GV对Hela细胞的毒性作用(P<0.01)。结论龙胆泻肝汤70%乙醇提物能显著抑制GV增殖,并能抑制GV细胞毒性、粘附能力和生物膜形成。AIM To study the inhibitory effects of Longdan Xiegan Decoction(LXD) on Gardnerella vaginalis(GV) in vitro.METHODS LXD was subjected to the following detections: its effect on GV proliferation by dilution method, and its minimum inhibitory concentration, its influence on GV adhesion to Hela cells by Gram staining, its effect on GV cytotoxicity by MTT assay, its effect on the formation of GV biofilm by 96-well microplate method, its impact on the expressions of GV BAP and sialidase mRNA by qRT-PCR.RESULTS The minimum inhibitory concentrations of aqueous extract, 70% ethanol extract and 90% ethanol extract of LXD on GV were identified to be(62.5±3.6),(15.6±1.5) and(125.0±2.8) mg/mL, respectively. The 70% ethanol extract of LXD(1.56, 15.6 mg/mL) significantly reduced the adhesion of GV to Hela cells, inhibited the formation of GV biofilm(P<0.01), down-regulated the expressions of BAP and sialidase(P<0.05, P<0.01), and the 70% ethanol extract LXD(15.6 mg/mL) inhibited the toxicity of GV on Hela cells(P<0.01).CONCLUSION 70% ethanol extract of LXD can significantly inhibit proliferation of GV, reduce the adhesion and cytotoxicity of GV on Hela cells, and restrain the GV biofilms formation.

关 键 词：龙胆泻肝汤 阴道加德纳菌 细菌性阴道病 生物膜 

分 类 号：R285.5[医药卫生—中药学]