纳尔逊海湾病毒μNS蛋白与σNS蛋白的表达分析  

Expression and analysis of Nelson Bay orthoreovirus μNS protein and σNS protein

作　　者：董晗 郑国峰[1] 张军[1] 李永刚 Han Dong;Guo-feng Zheng;Jun Zhang;Yong-gang Li(Department of Respiratory,the Fourth Affiliated Hospital of China Medical University,Shengyang,Liaoning 110005,China;Department of Pathogenic Microorganism,Jinzhou Medical University,Jinzhou,Liaoning 121000,China)

机构地区：[1]中国医科大学附属第四医院呼吸内科,辽宁沈阳110005 [2]锦州医科大学病原微生物教研室,辽宁锦州121000

出　　处：《中国现代医学杂志》2020年第2期22-26,共5页China Journal of Modern Medicine

摘　　要：目的研究呼肠孤病毒科纳尔逊海湾病毒非结构蛋白μNS与σNS在感染及转染细胞中的表达和分析。方法在感染细胞中,通过免疫荧光方法在共聚焦显微镜下观察μNS蛋白的表达,以及μNS和σNS共定位情况;同时通过转染质粒的方法观察非感染细胞中μNS和σNS的表达及共定位情况。结果μNS蛋白在感染细胞和单独转染质粒细胞中都形成包涵体样结构,σNS和μNS蛋白相互作用,σNS和μNS蛋白共定位存在于包涵体样结构内。结论μNS不仅可以在病毒感染细胞中,而且可以在单独转染的细胞中形成包涵体样结构,σNS可以与μNS相互作用,共定位于感染和转染细胞的包涵体样结构中。Objective To study the expression and analysis of Nelson Bay orthoreovirus non-structural protein μNS and σNS in infected and transfected cells. Methods The expression of μNS protein and the colocalization of μNS with another non-structural protein σNS were observed by immunofluorescence assay through confocal microscopy in infected cells. The expression and colocalization of μNS and σNS were observed in non-infected cells which were transfected by plasmids. Results μNS protein formed inclusion bodies in both infected and transfected cells. The σNS protein interacted with μNS protein. σNS and μNS protein were colocalized in inclusion bodies. Conclusion μNS can form inclusion bodies not only in virus-infected cells but also in cells transfected. σNS can interact and was colocalize with μNS in the inclusion bodies of infected and transfected cells.

关 键 词：病毒 蛋白 基因表达调控 病毒 

分 类 号：R563.19[医药卫生—呼吸系统]