日光性角化病中RUNX3和Bcl-2蛋白表达及其临床意义探讨  

Expression of RUNX3 and Bcl-2 Proteins in Solar Keratosis and Their Clinical Significance

作　　者：阚蓓[1] 韩莉[1] 张勇[1] 刘艳[1] 李钒[2] KAN Bei;HAN Li;ZHANG Yong;LIU Yan;LI Fan(Department of Dermatology,Chengdu Second People's Hospital,Chengdu 610000,Sichuan,China;Department of Pathology,Chengdu Second People's Hospital,Chengdu 610000,Sichuan,China)

机构地区：[1]成都市第二人民医院皮肤科,四川成都610000 [2]成都市第二人民医院病理科,四川成都610000

出　　处：《中国美容医学》2020年第1期58-61,共4页Chinese Journal of Aesthetic Medicine

摘　　要：目的:探讨日光性角化病中人类相关转录因子(RUNX3)、B细胞淋巴瘤-2基因(Bcl-2)蛋白表达及其临床意义。方法:分别对40例正常皮肤组织、40例日光性角化病皮肤组织及40例基底细胞癌组织进行RUNX3、Bcl-2检测,通过免疫组化分析RUNX3、Bcl-2阳性表达率,并分析二者的相关性。结果:基底细胞癌组织中RUNX3、Bcl-2阳性表达率分别为95.00%、92.50%高于日光性角化病皮损组织中二者的阳性表达率(80.00%、75.00%),且高于正常皮肤组织(57.50%、32.50%),差异有统计学意义(P<0.05)。RUNX3、Bcl-2在日光性角化病中共同表达阳性28例,共同表达阴性6例,经Spearman相关性分析,RUNX3、Bcl-2在日光性角化病中呈现正相关(r=0.577,P<0.05)。RUNX3、Bcl-2蛋白在肥厚型、萎缩型、鲍温样型、棘层松懈型、苔藓样型病理亚型日光性角化病中的阳性表达率比较差异无统计学意义(P>0.05)。结论:RUNX3、Bcl-2在日光性角化病组织中高表达,二者阳性表达率呈现正相关,且在不同病理亚型日光性角化病中的阳性表达率无差异。Objective To investigate expression of human runt-related transcription factor 3 (RUNX3) and B-cell lymphoma-2 gene (Bcl-2) in solar keratosis (SK) and their clinical significance.Methods R RUNX3 and Bcl-2 were detected in 40 normal skin tissues,40 actinic keratosis skin tissues and 40 basal cell carcinoma tissues respectively.Immunohistochemistry was performed to analyze positive expression rates of RUNX3 and Bcl-2,and their correlation.Results The positive expression rates of RUNX3 and Bcl-2 in basal cell carcinoma tissues were 95.00% and 92.50%,respectively,higher than those in SK skin lesion tissues (80.00%,75.00%),and higher than those in normal skin lesion tissues (57.50%,32.50%),the differencea were statistically significant(P<0.05).There were 28 cases with positive expression of RUNX3 and Bcl-2 in SK,and 6 cases with negative expression of the two.Spearman correlation analysis showed that RUNX3 was positively related with Bcl-2 in SK (r=0.577,P<0.05).There was no significant difference in positive expression rate of RUNX3 or Bcl-2 protein among SK of hypertrophic type,atrophic type,bowenoid type,nikolsky type and lichenification type (P>0.05).Conclusion RUNX3 and Bcl-2 are highly expressed in SK tissues.The positive expression rates of them are positively correlated.And there is no difference in positive expression rate of the two among SK with different pathological subtypes.

关 键 词：日光性角化病 基底细胞癌 RUNX3 Bcl-2 免疫组化法 阳性表达率 

分 类 号：R758.1[医药卫生—皮肤病学与性病学]