饮食失节对SD大鼠胃肠激素水平及肠道菌群的影响  

Effects of irregular diet on gastrointestinal peptide hormones and intestinal flora in SD rats

作　　者：李雁 王晓喜 陈琦 杜玉枝[1] 毕宏涛[1] 魏立新[1] 尚靖[1,3] LI Yan;WANG Xiao-xi;CHEN Qi;DU Yu-zhi;BI Hong-tao;WEI Li-xin;SHANG Jing(Qinghai Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810008,China;University of Chinese Academic of Sciences,Beijing 100049,China;College of Chinese Medicine,China Pharmaceutical University,Nanjing 210000,China)

机构地区：[1]中国科学院西北高原生物研究所,青海省藏药药理学与安全性评价研究重点实验室,青海西宁810000 [2]中国科学院大学,北京100049 [3]中国药科大学中药学院,江苏南京210000

出　　处：《甘肃农业大学学报》2019年第6期8-14,共7页Journal of Gansu Agricultural University

基　　金：国家重点研发计划项目(2018YFC1708006);青海省重点实验室专项(2017-ZJ-Y08)

摘　　要：【目的】观察饥饱失常法对SD大鼠胃肠激素水平以及肠道菌群的影响,初步探讨饮食失节在功能性消化不良发生中的作用及意义.【方法】将24只SD大鼠随机分为2组,正常饮食组(A)和饥饱失常组(B)各12只,B组单日进食,双日禁食,15 d后测定大鼠体质量、进食量、胃内甲基橙残留率、血浆胃动素(MTL)、胃促生长素(Ghrelin),一氧化氮(NO)含量以及肠道菌群多样性.【结果】15 d后,B组大鼠体质量小于A组(P<0.05),进食量(P<0.01)以及内甲基橙残留率(P<0.01)均高于A组;B组大鼠NO水平显著低于A组(P<0.01),Ghrelin水平显著高于A组(P<0.05),MTL含量低于A组,但无显著性差异(P>0.05);B组大鼠Lactobacillaceae(P<0.01),Bifidobacteriaceae(P<0.05),Enterobacteriaceae(P<0.05)菌群的相对丰度都显著高于A组,而Bacteroidales_S24-7_group(P<0.01),Ruminococcaceae(P<0.001),Verrucomicrobiaceae(P<0.05)菌群的相对丰度则显著低于A组.【结论】饮食失节能使SD大鼠胃肠激素水平以及肠道菌群多样性发生显著性变化,从而导致大鼠发生功能性消化不良病症.【Objective】To explore the effect of irregular diet on gastrointestinal peptide hormones and intestinal flora in SD rats.【Method】Rats were divided into two groups randomly,the control group(A)and the irregular diet group(B),give on the single day and ban on eating on the double day.The weight,the food intake and the Gastric methyl orange residue rate,the level of motilin(MTL),ghrelin in plasma and NO were measured in the antrum tissue and intestinal flora after 15 days.【Result】After experiments,the weight of rats in group B was lower than that in group A(P<0.05),the food intake(P<0.001)and the gastric methyl orange residue rate(P<0.001)were higher than group A.In addition the level of NO is lower in group B than A(P<0.01),Ghrelin in group B is higher than A(P<0.05).The abundance of intestinal flora in family level also changed significantly,including Lactobacillaceae(P<0.01),Bifidobacteriaceae(P<0.05),Enterobacteriaceae(P<0.05),Bacteroidales_S24-7_group(P<0.01),Ruminococcaceae(P<0.01),Verrucomicrobiaceae(P<0.05).【Conclusion】Irregular diet can change the level of gastrointestinal peptide hormones and intestinal flora in rats,leading to functional dyspepsia in rats,which indicates that dietary factors are important cause of FD occurrence and development,and the impact is greater.

关 键 词：饮食失节 胃动素 胃促生长素 肠道菌群 

分 类 号：Q48[生物学—生理学]