枳椇子降尿酸功效成分  

Uric acid-lowering ingredients of Semen Hoveniae

作　　者：于俊俊 徐德平[1] YU Junjun;XU Deping(School of Science and Technology,Jiangnan University,Wuxi 214122)

机构地区：[1]江南大学食品学院,无锡214122

出　　处：《食品科技》2019年第11期227-231,237,共6页Food Science and Technology

基　　金：中央高校基本科研业务费专项(JUSRP21127)

摘　　要：研究枳椇子降尿酸活性成分,为枳椇子的开发应用提供基础。采用乙醇提取,大孔树脂、MCI、ODS等多种层析方法分离,经高尿酸动物模型对有效部位筛选活性成分,核磁共振鉴定化学结构。结果表明:枳椇子醇提物经大孔树脂分离得到30%乙醇洗脱物作用效果最明显,该部分经进一步分离得到3个化合物,分别是3,5,3’,4’,5’-五羟基二氢黄酮醇(Ⅰ)、杨梅素(Ⅱ)、二氢杨梅素(III)。三者均能够抑制黄嘌呤氧化酶,半抑制浓度(Half maximal inhibitory concentration,IC50)分别为12.80、15.36、19.56μg/m L,其中化合物Ⅰ为首次从枳椇子中分离得到并证实具有降尿酸作用。因此:二氢异黄酮是枳椇子降尿酸功效成分。The research on the active components of Semen Hoveniae in reducing uric acid will provide the basis for the development and application of Semen Hoveniae. Ethanol extraction, macroporous resin, MCI, ODS and other chromatography methods were used to separate the active components, and the chemical structure was identified by NMR. The results showed that 30% ethanol eluate from Semen Hoveniae by macroporous resin had the most obvious effect. Three compounds were obtained by further separation, namely, 3,5,3’,4’,5’-pentahydroxydihydroflavonol(I), myricetin(II), dihydromyricetin(III). All of them can inhibit xanthine oxidase, and the semi inhibitory concentration(IC50) is 12.80 μg/mL, 15.36 μg/mL and 19.56 μg/mL, respectively. Compound I was isolated from Semen Hoveniae for the first time and proved to have the effect of reducing uric acid. Therefore, dihydroisoflavone is an effective component of Semen Hoveniae.

关 键 词：枳椇子 降尿酸 杨梅素 二氢杨梅素 3 5 3’ 4’ 5’-五羟基二氢黄酮醇 分离 鉴定 

分 类 号：R284.2[医药卫生—中药学]