质谱条件对蛋白质ESI-MS图谱荷电状态分布的影响  

Effect of MS instrumental settings on charge state distribution of protein ESI mass spectra

作　　者：张悦 刘少贤 樊娟[1] 张延妮[1] 岳宣峰[1] ZHANG Yue;LIU Shao-xian;FAN Juan;ZHANG Yan-ni;YUE Xuan-feng(Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province,Shaanxi Normal University,Xi'an 710062)

机构地区：[1]陕西省生命分析化学重点实验室陕西师范大学化学化工学院

出　　处：《分析试验室》2019年第12期1392-1396,共5页Chinese Journal of Analysis Laboratory

基　　金：中央高校基本科研业务费(GK201703078,GK201806003);陕西省重点研发计划(2018SF-287)资助

摘　　要：以脱辅基肌红蛋白质和还原性溶菌酶为研究对象,考察了射频透镜1电压等质谱条件对蛋白质ESI-MS图谱荷电状态分布(CSD)的影响。研究发现:在“较软”ESI条件下射频透镜1电压能在一定程度上显著改变ESI-MS图谱右左两侧两个分布的相对强度,而右左两侧两个电荷中心的相对位置基本保持不变,这种影响与溶液相中蛋白质分子的构象类型密切有关,本文对这一现象的机理做了探讨。Apomyoglobin and reduced lysozyme were chosen as the research objects,and the effects of MS instrumental parameters such as RF Lens 1 voltage on charge state distribution(CSD)of ESI-MS spectrum were investigated.It was found that under the condition of"soft"ESI instrumental settings,the voltage of RF lens 1 could significantly change the relative intensity of the two distributions on the right and left sides of ESI-MS spectrum,while the relative positions of the two charge centers on the right and left sides remained basically unchanged.The effect of RF Lens 1 voltage was closely related to the conformation of proteins in the bulk solution,and the mechanism was discussed.

关 键 词：电喷雾离子源质谱 蛋白质 质谱条件 荷电状态分布 

分 类 号：O657.63[理学—分析化学]