甲烷氧化菌素功能化纳米金可视化检测微量铜  

Visual detection of trace copper ions by methanobactin functionalized gold nanoparticles

作　　者：刘丰源 辛嘉英[1,2] 孙立瑞 夏春谷[2] LIU Feng-yuan;XIN Jia-ying;SUN Li-rui;XIA Chun-gu(Key Laboratory for Food Science and Engineering,Harbin University of Commerce,Harbin 150076;State Key Laboratory for Oxo Synthesis and Selective Oxidation,Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,Lanzhou 730000)

机构地区：[1]哈尔滨商业大学食品科学与工程重点实验室,哈尔滨150076 [2]中国科学院兰州化学物理研究所羰基合成与选择氧化国家重点实验室,兰州730000

出　　处：《分析试验室》2019年第12期1495-1498,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(21573055)资助

摘　　要：建立了基于甲烷氧化菌素(Mb)功能化纳米金(GNPs)可视化检测微量Cu2+的方法。利用Mb还原氯金酸一步合成了Mb功能化的GNPs,其在Cu 2+诱导下会发生聚集,聚集过程可通过紫外-可见光谱来监控。发现吸光度比A 654/A 520与Cu 2+浓度在1~4μmol/L呈良好的线性关系(R2=0.98337),检出限为0.34μmol/L。A method for visually detecting trace copper ions based on methanogen(Mb)functionalized GNPs was developed.The Mb functionalized GNPs were synthesized in one-step Mb reduction of chloroauric acid.The Mb functionalized GNPs could aggregate under the induction of copper ions and the aggregation process could be monitored by ultraviolet-visible spectroscopy.It was found that the absorbance ratio of A 654 to A 520 gave a linear response with copper ion concentration in the range of 1-4μmol/L(R 2=0.98337)with the detection limit of 0.34μmol/L.

关 键 词：甲烷氧化菌素功能化纳米金 铜离子 可视化检测 

分 类 号：O657.3[理学—分析化学] R991[理学—化学]