三烯霉素J——来自深海细菌Ochrobactrum sp.的新安莎霉素  

Trienomycin J, a new ansamycin from deep-sea derived bacterium Ochrobactrum sp.

作　　者：王聪 崔同旭[2] 王冬阳 朱伟明[2,3] WANG Cong;CUI Tong-xu;WANG Dong-yang;ZHU Wei-ming(Guangxi Key Laboratory of Chemistry and Engineering of Forest Products,Key Laboratory of Guangxi Colleges and Universities for Food Safety and Pharmaceutical Analytical Chemistry,School of Chemistry and Chemical Engineering,Guangxi University for Nationalities,Nanning 530006,China;Key Laboratory of Marine Drugs,Ministry Education of China,School of Pharmacy,Ocean University of China,Qingdao 266003,China;Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266003,China)

机构地区：[1]广西林产化学与工程重点实验室,广西高校食品安全与药物分析化学重点实验室,广西民族大学化学化工学院,广西南宁530006 [2]海洋药物教育部重点实验室,中国海洋大学医药学院,山东青岛266003 [3]青岛海洋科学与技术试点国家实验室,山东青岛266003

出　　处：《中草药》2019年第23期5661-5665,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目（U1501221）;国家自然科学基金资助项目（41876172）;广西自然科学基金青年科学基金项目（2018JJB150106）

摘　　要：目的探究深海来源细菌Ochrobactrum sp.OUCMDZ-2164中的细胞毒活性代谢产物。方法利用硅胶柱色谱、凝胶柱色谱和高效液相色谱等手段对发酵产物进行分离纯化,运用紫外、红外、质谱、核磁共振及圆二色谱等方法鉴定化合物的结构,采用MTT和细胞计数试剂盒-8(CCK-8)法分别评价化合物对乳腺癌MCF-7细胞和肺癌A549细胞及慢性髓性白血病K562细胞的增殖抑制活性。结果从细菌Ochrobactrum sp.OUCMDZ-2164的发酵产物中分离获得了4个化合物,分别鉴定为3-O-去甲基三烯霉醇(1)、flazin(2)、flazin-3-carboxylicacid(3)和胸腺嘧啶(4)。化合物1在10μmol/L浓度下对MCF-7细胞的增殖抑制率为61.5%。结论化合物1为新的安沙霉素类化合物,命名为三烯霉素J,其具有一定的乳腺癌细胞毒活性。Objective To identify the cytotoxic natural products from the deep-sea derived Ochrobactrum sp.OUCMDZ-2164.Methods The isolations and purifications of compounds were performed by means of column chromatography over silica gel and Sephadex LH-20 as well as HPLC.Their structures were elucidated through the analysis of UV,IR,MS,NMR and ECD spectra.The cytotoxicities against MCF-7,A549 and K562 cells were evaluated by MTT and CCK-8 methods.Results From the fermentation broth of Ochrobactrum sp.OUCMDZ-2164,we isolated and identified four compounds(1-4).Compound 1 was identified as a new ansamycin and named trienomycin J,and the structures of 1-4 were identified as 3-O-demethyltrienomycinol,flazin,flazin-3-carboxylic acid and thymine,respectively.Compound 1 showed cytotoxic effect on the MCF-7 cells with 61.5%inhibition rate at 10μmol/L.Conclusion Compound 1 was a new ansamycin named trienomycin J,with cytotoxic activity against human breast cancer cells(MCF-7).

关 键 词：深海细菌 苍白杆菌 安沙霉素类化合物 乳腺癌细胞毒活性 三烯霉素J 

分 类 号：R284.1[医药卫生—中药学]