海藻羊栖菜全草化学成分研究  

Study on chemical constituents from whole herbs of Sargassum fusiforme

作　　者：王思玉 向俊 汪玉梅[1] 韦霞 冯婵 范倩 张翠仙[1] WANG Si-yu;XIANG Jun;WANG Yu-mei;WEI Xia;FENG Chan;FAN Qian;ZHANG Cui-xian(College of Traditional Chinese Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Pharmaceutical Department,Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510120,China)

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]广州中医药大学第二附属医院药剂科,广东广州510120

出　　处：《中草药》2019年第23期5670-5676,共7页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目（81741160）;广东省科技计划（2017A020217008）;广东省科技计划（2015A020216017）;广州中医药大学“薪火计划”重点项目（XH20170110）;海南大学南海海洋资源利用国家重点实验室开放课题（2016003）;广东省自然科学基金项目（2014A030313411）

摘　　要：目的研究海洋中药羊栖菜Sargassumfusiforme全草的化学成分。方法采用硅胶、MCI和ODS及半制备HPLC等柱色谱技术方法进行分离纯化;采用现代波谱技术(NMR、MS、ORD等)并与文献物理常数对照等方法确定化合物的结构。结果从羊栖菜全草的50%乙醇提取物的醋酸乙酯萃取部分中分离得到19个化合物,依次为羽扇豆酮(1)、鲍尔山油柑烯酮(2)、β-香树脂酮(3)、α-香树脂酮(4)、黑麦草内酯(5)、异黑麦草内酯(6)、(3S,5R,6S,7E)-5,6-环氧-3-羟基-7-大柱香波龙烯-9-酮(7)、(R)-去氢催吐萝芙醇(8)、24-乙基-4,24(28)-胆甾二烯-3-酮(9)、岩藻甾醇(10)、29-氢过氧基豆甾-5,24(28)-二烯-3β-醇(11)、24-氢过氧基-24-乙烯基胆甾醇(12)、马尾藻甾醇(13)、(24R)-豆甾-5,28-二烯-3β,24-二醇-7-酮(14)、二氢槲皮素(15)、金色酰胺醇乙酸酯(16)、邻苯二甲酸二丁酯(17)、单棕榈酸甘油酯(18)、棕榈酸甲酯(19)。结论从羊栖菜全草中分离并确定的化合物结构类型,涉及三萜及倍半萜类、高度氧化甾醇和生物碱等化合物,其中化合物1~4、7、8、14~19均首次从该种植物中分离得到。Objective To study the chemical constituents of the whole herbs of Sargassum fusiforme.Methods The chemical constituents from S.fusiforme were separated and purified by silica gel,MCI,ODS,Sephadex LH-20 gel column chromatographies,and preparative HPLC.Their structures were identified by analysis of NMR,MS,ORD,etc,and comparing physicochemical properties of compounds with references.Results A total of 19 compounds were obtained from the 50%ethanol-H2O extract of S.fusiforme,and their structures were determined as lupenone(1),bauerenone(2),β-amyrenone(3),α-amyrenone(4),loliolide(5),isololiolide(6),(3S,5R,6S,7E)-5,6-epoxy-3-hydroxy-7-megastigmen-9-one(7),(R)-dehydrovomifoliol(8),24-ethylcholesta-4,24(28)-dien-3-one(9),fucosterol(10),29-hydroperoxystigmasta-5,24(28)-dien-3β-ol(11),24-hydroperoxy-24-vinylcholesterol(12),saringosterol(13),(24R)-5,28-stigmastadiene-3β,24-diol-7-one(14),dihydroquercetin(15),aurantiamide acetate(16),dibutyl phthalate(17),glycerol monopalmitate(18),and methyl hexadecanoate(19).Conclusion The compounds isolated from S.fusiforme were mainly identified as triterpenoids,sesquiterpenoids,highly oxidized sterols,alkaloids and so on.Compounds 1-4,7-8 and 14-19 are isolated from S.fusiforme for the first time.

关 键 词：羊栖菜 次生代谢产物 三萜 倍半萜 甾醇 羽扇豆酮 β-香树脂酮 二氢槲皮素 

分 类 号：R284.1[医药卫生—中药学]