Box-Behnken响应面法优化超滤与络合萃取反萃取技术耦合制备甘草素工艺的研究  

Box-Behnken response surface method optimized technological research of ultrafiltration-complexation extraction and back extraction technology to prepare liquiritigenin

作　　者：石盘棋 刘晓霞[1] 孙晓燕[1] 王继龙[1] 魏舒畅[1] 周博 蒲红利 SHI Pan-qi;LIU Xiao-xia;SUN Xiao-yan;WANG Ji-long;WEI Shu-chang;ZHOU Bo;PU Hong-li(Research Center of traditional Chinese Medicine Pharmaceutical Technology and Engineering of Gansu Province,Gansu University of Chinese Medicine,Lanzhou 730000,China)

机构地区：[1]甘肃中医药大学甘肃省中药制药工艺工程研究中心

出　　处：《中草药》2019年第23期5739-5744,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目（81460608）;甘肃省基础研究创新群体项目（1506RJIA034）

摘　　要：目的建立一种从甘草超滤液中制备甘草素的工艺路线。方法以甘草素保留率为指标,采用U5(53)均匀设计优化超滤工艺;以萃取率为指标,采用Box-Behnken响应面法优化络合萃取工艺;以甘草素反萃取率为指标,确定反萃取甘草素的工艺条件。结果甘草素的最佳超滤条件为孔径10 nm的无机陶瓷膜、压力0.12 MPa、料液温度25℃,甘草素保留率98.74%;1%三烷基氧化膦(TRPO)、萃取10 min、5 mL有机相溶液为最佳络合萃取条件,甘草素萃取率99.44%;12.5 mmol/L NaOH水溶液为最佳反萃取剂,反萃取率98.88%。结论作为一种新兴的中药萃取技术,超滤-络合萃取反萃取技术具有高选择、高效、萃取剂循环利用等优点,可为甘草素的研究提供一种全新的制备技术。Objective A technological route was established for preparing liquiritigenin from the licorice ultrafiltrate.Methods U5(53)uniform design was used to optimize the ultrafiltration process with the retention rate of liquiritigenin as the index;Box-Behnken response surface method was used to optimize the complexation extraction process with the extraction rate of liquiritigenin as the index;The technological condition of back extraction of liquiritigenin was determined by the liquiritigenin stripping rate.Results The optimal ultrafiltration conditions for liquiritigenin were inorganic ceramic membranes with a pore size of 10 nm,a pressure of 0.12 MPa,and solution temperature of 25℃,retention rate of liquiritigenin was 98.74%.Optimum complexation extraction conditions were as following:1%TRPO complexing agent,extraction for 10 min in 5 mL organic phase solution,the extraction rate of liquiritigenin was 99.44%;The 12.5 mmol/L NaOH aqueous solution was the best back-extractant,and the back extraction rate was 98.88%.Conclusion As a new Chinese medicine extraction technology,ultrafiltration-complexation extraction and back extraction technology have the advantages of high selectivity,high efficiency,and recycling of extractant,which can provide a new preparation technology for the research of liquiritigenin.

关 键 词：甘草素 超滤 络合萃取 反萃取 三烷基氧化膦 磺化煤油 响应面法 

分 类 号：R284.2[医药卫生—中药学]