天麻钩藤饮加减通过抑制NF-κB表达和核转位缓解高血压性脑出血肝阳化风证大鼠神经损伤的研究  

Tianma Gouteng Decoction Can Alleviate Nerve Injury in Rats with Hypertensive Intracerebral Hemorrhage by Inhibiting NF-κB Expression and Nuclear Translocation

作　　者：袁春云[1] 袁思斯 伍大华[1] 刘春华[2] YUAN Chunyun;YUAN Sisi;WU Dahua;LIU Chunhua(Hunan Institute of Traditional Chinese Medicine,Changsha 410006,Hunan,China;Hunan University of Chinese Medicine,Changsha 410208,Hunan,China)

机构地区：[1]湖南省中医药研究院附属医院,湖南长沙410006 [2]湖南中医药大学,湖南长沙410208

出　　处：《中华中医药学刊》2019年第12期2829-2832,I0023,I0024,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81874462);湖南省自然科学基金项目(2016JJ6084);湖南省中医药管理局科学研究项目(201662);湖南省科卫联合基金项目(2018JJ6107)

摘　　要：目的:探讨天麻钩藤饮加减对高血压性脑出血肝阳化风证大鼠神经保护作用。方法:建立急性期高血压性脑出血的肝阳化风证大鼠模型,随机分组为模型组、西药组、天麻钩藤饮组合中西结合组,同步设空白组和假手术组。给予相应干预后,处理动物。对大鼠神经缺损症状、脑组织含水量、组织形态学、脑组织中NF-κB的mRNA含量和蛋白含量以及核转位情况进行检测。结果:天麻钩藤饮加减及其联合西药,对高血压性脑出血肝阳化风证大鼠神经缺损症状有明显改善,可明显下调模型大鼠脑组织含水量,同时可明显减少NF-κB的mRNA表达和蛋白表达,以及抑制NF-κB的核转位情况,且中西结合组效果优于单用组。结论:天麻钩藤饮加减与甘露醇及速尿联合对急性期高血压性脑出血的肝阳化风证模型大鼠的脑出血后的组织损伤及脑水肿,保护神经功能,改善预后,可能与下调NF-κB的mRNA表达和蛋白表达,以及抑制NF-κB的核转位情况关系密切。Objective:To explore the neuroprotective effect of Tianma Gouteng Decoction on rats with hypertensive intracerebral hemorrhage(H)and establish the model of liver Yang leading to internal wind syndrome of acute hypertensive cerebral hemorrhage.Methods:The rat model of liver Yang leading to internal wind induced by hypertensive intracerebral hemorrhage was established and randomly divided into the model group,the western medicine group and the combination group of Tianma Gouteng Decoction,and the blank group and the sham operation group were set up simultaneously.The corresponding intervention was given and the animals were treated.The symptoms of nerve defect,water content of brain tissue,histomorphology,mRNA content and protein content of NF-κB in brain tissue and nuclear transposition were detected in the rats.Results:Tianma Gouteng Decoction and its combination with western medicine can obviously improve the symptoms of nerve defect in rats with hypertensive cerebral hemorrhage of liver Yang leading to internal wind sydnrome and can obviously reduce the water content of the brain tissue of the model rats,reduce the mRNA expression and protein expression of NF-κB as well as inhibit the nuclear transposition of NF-κB.The effect was better.Conclusion:The combination of Tianma Gouteng Decoction combined with mannitol and furosemide on the tissue injury and brain edema after cerebral hemorrhage in the rat model can protect nerve function and improve the prognosis,which may be related to the down regulation of the mRNA expression and protein expression of NF-κB as well as the inhibition of the nuclear transfer of NF-κB.

关 键 词：天麻钩藤饮 高血压性脑出血 肝阳化风证 神经缺损症状 核转录因子κB 

分 类 号：R285.5[医药卫生—中药学]