针刀干预对颈椎病兔颈后伸肌组织学和超微结构的影响  

Effect of Acupotomy on Histopathology and Ultrastructure of Posterior Extensor Cervical Muscle Cells in Cervical Spondylosis Rabbits

作　　者：刘福水[1] 方婷[1] 赵梅梅[1] 周凡媛 陈梅[1] 游建宇 金玉立 谢金梅 UU Fushui;FANG Ting;ZHAO Meimei;ZHOU Fanyuan;CHEN Mei;YOU Jianyu;JIN Yuli;XIE Jinmei(Clinical Medical College of Jiangxi University of Chinese Medicine,Nanchang 430000,Jiangxi,China)

机构地区：[1]江西中医药大学临床学院

出　　处：《中华中医药学刊》2019年第12期2870-2873,I0033,I0034,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81560792);江西省教育厅科学技术研究项目(GJJ170702);江西省卫生与计划生育委员会中医药科研项目(2016Z03);江西省卫生与计划生育委员会科技计划项目(20185514)

摘　　要：目的:观察针刀干预对颈椎病兔颈后伸肌细胞病理和超微结构的影响。方法:40只新西兰雄性兔按随机数字表随机分为空白组、模型组、电针组、针刀组,每组各10只,空白组不做干预处理,其余建立颈椎病动物模型,持续12周。造模成功后针刀组于兔颈肌硬结、条索以及棘突等处行针刀干预,每周1次,共3次;电针组于兔颈肌两侧"天柱""颈百劳""大杼"穴处行毫针干预,隔天治疗1次,每周3次,共3周。干预结束后1周取兔颈后两侧后伸肌,HE染色法在普通光镜下观察颈肌形态学改变,电镜下观察颈肌组织的超微结构变化。结果:光镜下模型组肌纤维排列紊乱,染色不均,甚至断裂,细胞核分布紊乱,出现大量炎性细胞。电镜下模型组肌丝溶解、变性,线粒体广泛水肿、空泡化,可见大量自噬体和凋亡小体。经针刀及电针干预后,兔颈肌整体情况明显改善,且针刀组优于电针组,针刀组肌纤维排列整齐,可见肌卫星细胞,线粒体分布均匀。结论:针刀疗法可改善兔颈部受损肌肉病理及超微结构变化,促进劳损颈肌再生修复,这可能是针刀治疗颈椎病的部分作用机制。Objective:To explore the effect of acupotomy on histopathology and ultrastructure of posterior cervical extensor muscles cells in cervical spondylosis(CS)rabbits.Methods:Forty New Zealand male rabbits were randomly divided into normal control,model,electro-acupuncture(EA),and acupotomy groups(n=10).No intervention was given in the control group,and the other groups established the CS animal model for 12 weeks.After the model was successfully made,acupotomy was performed at the point of indurate,cord-like mass and spinous process,once a week for three weeks.For EA group,EA was applied to bilateral"Tianzhu"(BL10),"Jingbailao"(EX-HN15)and"Dazhu"(BL11)of the rabbit neck muscle,once a day,three times a week for 3 weeks.One week after the end of intervention,the posterior extensor muscles were taken out from bilateral rabbits neck muscles.The changes of HE and ultrastructure of cervical muscle were observed under light microscope and electronic microscope respectively.Results:Under the light microscope,the muscle fibers were disordered,stained unevenly,even ruptured,the nuclear distribution was disordered,and a large number of inflammatory cells appeared in the model group.Under electron microscopy,myofilolysis,degeneration,extensive mitochondrial edema and vacuolation,and a large number of autophagy and apoptotic bodies were observed in the model group.After acuputomy and EA intervention,the whole condition of rabbit neck muscle was improved obviously,and the acupotomy group was superior to the EA group.In acupotomy group,muscle fiber was arranged neatly,muscle satellite cells could be seen,and mitochondria was evenly distributed.Conclusion:Acupotomy therapy can improve the histopathology and ultrastructural changes of the injured neck muscles and promote the regeneration and repair of the damaged neck muscles,which may be part of the mechanism of acupotomy in the treatment of cervical spondylosis.

关 键 词：针刀 颈椎病 颈肌 组织学 超微结构 

分 类 号：R245.3[医药卫生—针灸推拿学] R681.55[医药卫生—中医临床基础]