姜黄素的提取方法研究  

Study on the extraction method of curcumin

作　　者：王斌[1] 梁婷婷 石恩娴[2] 张建[1] 王宁[1] 吕俊杰[1] 李丽红 卫建琮[1] WANG Bin;LIANG Ting-ting;SHI En-xian;ZHANG Jian;WANG Ning;LYU Jun-jie;LI Li-hong;WEI Jian-cong(Basic Medical College of Shanxi Medical U niversity,Taiyuan 030001,China;College of Pharmacy,Shanxi Medical U niversity,Taiyuan 030001,China)

机构地区：[1]山西医科大学基础医学院,山西太原030001 [2]山西医科大学药学院,山西太原030001

出　　处：《分子科学学报》2019年第6期478-483,I0002,共7页Journal of Molecular Science

基　　金：国家自然科学基金资助项目(21705104);山西医科大学博士启动基金资助项目(03201601)

摘　　要：借助微波辅助手段,加入新制的系列咪唑类离子液体(ILs),利用正交法设计实验并优化了不同因素水平下生姜中姜黄素的提取工艺,利用其对1,1-二苯基-2-三硝基苯肼(DPPH)的清除率检测了姜黄素的抗氧化性.最佳提取工艺条件为:离子液体(溴化1-甲基-3-正癸基咪唑)浓度为1.5mol·L-1,乙醇体积分数为80%,料液比为1∶15,微波功率为165 W,微波时间为20 min,最终提取率为0.426%.此条件下姜黄素对DPPH自由基的清除率为65.07%,IC50为52.8μg·L-1,表明姜黄素有较强的抗氧化性.A novel microwave-assisted method for extractingcurcumin from ginger has been developed by employing imidazolium ionic liquid as the extraction solvent and optimizing the extracting conditions with orthogonal experiments.Bromide 1-methyl-3-n-decyl imidazole exhibited the best performance among a series of synthesized imidazolium ionic liquids.The optimal extraction rate could be achieved by microwaving(solid-liquid ratio of 1∶15)at 165 Wfor 20 min,with 1.5 mol·L-1 of bromide 1-methyl-3-n-decyl imidazole and 80%percentage of ethanol(V/V)as the extracting solvent.And the average extraction yield was 0.426%.Moreover,the oxidation resistance of the obtained curcumin was evaluated by determiningthe 1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl(DPPH)clearance.Under the optimized conditions,the extracted curcuminshoweda scavenging rate of 65.07%(IC50:52.8μg·L-1)to DPPH,indicating a strong antioxidant capacity.

关 键 词：姜黄素 离子液体 DPPH 

分 类 号：O655[理学—分析化学]