表达鹅细小病毒VP3基因的重组新城疫病毒安全性及免疫效果评价  

Evaluation of safety and immune effect of recombinant Newcastle disease virus expressing VP3 gene of goose parvovirus

作　　者：钦琪 肖跃强[2] 王建忠[3] 徐小洪 丁佳欣 李金斗 王玮琦 丁壮 QIN QI;XIAO Yue-qiang;WANG Jian-zhong;XU Xiao-hong;DING Jia-xin;LI Jin-dou;WANG Wei-qi;DING Zhuang(Key Laboratory of Zoonosis Research,Ministry of Education,College of Veterinary Medicine,Jilin University,Changchun130062,China;Shandong Binzhou Animal Science&Veterinary Medicine Academy,Binzhou,Shandong 256600,China;College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林大学动物医学学院人兽共患病研究教育部重点实验室,吉林长春130062 [2]山东省滨州畜牧兽医研究院,山东滨州256600 [3]吉林农业大学动物科学技术学院,吉林长春130118

出　　处：《中国兽医学报》2019年第12期2315-2321,共7页Chinese Journal of Veterinary Science

基　　金：国家重点研发计划资助项目(2018YFD0501400)

摘　　要：新城疫(Newcastle disease,ND)和鹅细小病毒病(goose parvovirus infection,GP)对养鹅业产生了严重危害,为了防控这2种疾病,利用反向遗传操作系统构建表达鹅细小病毒VP3基因的重组新城疫病毒rmNA-VP3,通过超剂量接种试验及攻毒保护试验对重组病毒rmNA-VP3进行安全性检测及免疫效果评价。结果可见,rmNA-VP3超剂量接种雏鹅后,未引起雏鹅胃及肠组织的病变;攻毒后rmNA-VP3能够有效诱导机体的免疫应答,产生较高滴度的抗体,对雏鹅产生100%的保护,并且rmNA-VP3能够快速清除雏鹅体内强毒,缩短排毒时间,抑制病毒复制及在鹅体内的扩散,这为以rmNA-VP3为基础的ND和GP联合防控提供了有效的试验依据。Newcastle disease(ND)and goose parvovirus infection(GP)have caused serious harm to the goose industry.In order to prevent and control these two diseases,a recombinant Newcastle disease virus rmNA-VP3expressing the goose parvovirus VP3gene was constructed using a reverse genetic engineering system.This study used re-dosing test and challenge protection to test the safety and immune effect of recombination virus rmNA-VP3.The results showed that rmNAVP3did not cause lesions in the stomach and intestine tissues after inoculation of goslings.After challenge,rmNA-VP3can effectively induce the body immune response,produce higher titers of antibodies,and produce 100%protection for goslings,and rmNA-VP3can quickly remove the violent virus from the poisonous gosling body,shorten the detoxification time,inhibit the virus replication and spread in the goose body,which provides effective prevention and control of ND and GP based on rmNA-VP3experiment.

关 键 词：新城疫病毒 鹅细小病毒 VP3基因 安全性 免疫效果 

分 类 号：S852.65[农业科学—基础兽医学]