基于转录组测序的奶牛泌乳初期与干奶期肝脏差异表达基因分析  

Differentially expressed genes in liver of Chinese Holstein cows between early lactation and dry period based on transcriptomes with high-throughput RNA-seq technology

作　　者：李茜[1,2] 李妍[3] 高艳霞[1] 孙东晓[4] 李建国[1] LI Qian;LI Yan;GAO Yan-xia;SUN Dong-xiao;LI Jian-guo(College of Animal Science and Technology,Hebei Agricultural University,Baoding,Hebei 071001,China;Hebei Animal Husbandry and Veterinary Institute,Baoding,Hebei 071000,China;College of Veterinary Medicine,Hebei Agricultural University,Baoding,Hebei 071001,China;College of Animal Science and Technology,China Agricultural University,Beijing100193,China)

机构地区：[1]河北农业大学动物科技学院,河北保定071001 [2]河北省畜牧兽医研究所,河北保定071000 [3]河北农业大学动物医学院,河北保定071001 [4]中国农业大学动物科技学院,北京100193

出　　处：《中国兽医学报》2019年第12期2458-2466,共9页Chinese Journal of Veterinary Science

基　　金：国家现代农业(奶牛)产业技术体系建设专项资金资助项目(CARS-36);河北省科技计划资助项目(16226604D)

摘　　要：旨在通过对奶牛干奶期和泌乳初期肝脏组织进行转录组测序和生物信息学分析,探明其不同生理阶段的差异表达基因和差异代谢通路。选取305d产奶量接近、干奶天数一致、怀孕天数接近的中国荷斯坦奶牛3头,分别在干奶期(产前50d)和泌乳初期(产后15d)活体采集肝脏组织,利用Illumina Hiseq^TM2500高通量RNA-seq测序技术对干奶期和泌乳初期肝脏RNA测序,使用TopHat2软件将测序得到的reads序列与牛(UMD3.1.66)参考基因组序列比对,在GO和KEGG数据库中进行聚类分析和富集分析。结果显示,泌乳初期和干奶期肝脏中差异表达基因共有409个,与干奶期相比,泌乳初期表达上调的基因有235个,下调的基因有174个。GO功能分类注释到生物学过程、细胞组成和分子功能数据库中的GO条目分别有63,41,15条。注释到KEGG的显著富集通路有21条(P<0.05)。该研究结果为挖掘奶牛产奶性状的候选基因提供了技术依据。The aim of this study was to find out the differentially expressed genes and metabolism pathways between early lactation and dry period in liver of Chinese Holstein cows by RNA-sequencing and bioinformatics analysis.Three Chinese Holstein cows were selected based on their similar 305-day milk yield,milk protein percentage and milk fat percentage,according to their monthly dairy herd improvement(DHI)test-day records of the first lactations.Liver biopsies were collected on 50-d before parturition(dry period)and 15-d after parturition(early lactation).The liver transcriptomes were analyzed by Illumina HiSeq^TM2500high-throughput RNA sequencing system.All RNA-seq reads were mapped on the reference bovine genome(UMD3.1.66)using TopHat2software.All genes were annotated using GO and KEGG databases.The results showed that there were 409differentially expressed genes in the two periods,in which 235genes were up-regulated and 174genes were down regulated in early period vs dry period.There were 63,41,15GO terms annotated into database of biological process,cellular component,molecular function in GO databases,respectively;there were 21KEGG terms significant pathways enriched(P<0.05).This study provides a technical base for further investigation of candidate genes for dairy milk production traits.

关 键 词：中国荷斯坦牛 转录组 高通量RNA测序 差异表达基因 代谢通路 肝脏 

分 类 号：S813.3[农业科学—畜牧学]