烟草Hsp70基因家族的鉴定及叶肉内CpHsp70基因的表达分析  

Identification of Tobacco Hsp70 Gene Family and Expression Analysis of CpHsp70 Gene in Mesophyll

作　　者：陈二龙[1,3] 张明刚 李成刚 孙占伟 杨晓亮 宋朝鹏[1] 申洪涛[3] CHEN Erlong;ZHANG Minggang;LI Chenggang;SUN Zhanwei;YANG Xiaoliang;SONG Zhaopeng;SHEN Hongtao(College of Tobacco Science,Henan Agricultural University,Zhengzhou 450002,China;China Tobacco Hubei Industrial Co.,Ltd.,Wuhan 430040,China;China Tobacco Henan Industrial Co.,Ltd.,Zhengzhou 450016,China)

机构地区：[1]河南农业大学烟草学院,郑州450002 [2]湖北中烟工业有限责任公司,武汉430040 [3]河南中烟工业有限责任公司,郑州450016

出　　处：《吉林农业大学学报》2019年第5期553-562,共10页Journal of Jilin Agricultural University

基　　金：河南中烟科技创新项目(ZW2015006);湖北中烟工业有限责任公司项目(2018A011YL05);河南省高等学校青年骨干教师项目(2015GGJS-079)

摘　　要：以NCBI和SGN数据库为基础,使用生物信息学技术对烟草Hsp70基因家族的成员进行鉴定,且以烟草品种“红花大金元”为材料,运用实时荧光定量PCR法,分析在烟草叶肉内发挥作用的CpHsp70基因成员在高温的逆境环境的表达量。结果表明:烟草含有61个Hsp70基因成员,其理化特性存在差异;染色体定位显示,Hsp70基因分布在18条染龟体中,其中叶绿体成员分布在6、17、19号染色体;系统进化显示,烟草Hsp70基因分布在5组,2亚组,叶绿体成员位于V-A亚组;基因结构显示,内含子数为0~13,叶绿体成员内含子数为7;保守基序显示,Motif在各组之间具有差异,特异性的Motif 16、Motif 17、Motif 20位于I组的细胞质成员中,M otif2、Motif 5属于ID组细胞质和IV组内质网成员中,Motif 15和Motif 19存在V-A亚组叶绿体成员中;实时荧光定量PCR分析呈现,NtCpHsp70基因不易受高温诱导。综上,烟草Hsp70功能结构具有多样性,而NtCpHsp70基因表达受烘烤环境的影响较小。Based on NCBI and SGN databases,bioinformatics techniques were used to identify members of tobacco Hsp70 gene family.Using tobacco variety"Hongda"as material,expression of CpHsp70 gene in tobacco mesophyll in high temperature environment was analyzed by real-time fluorescence quantitative PCR.The results showed that the tobacco contains 61 Hsp70 gene members with different physicochemical properties;chromosomal localization showed that Hsp70 gene was distributed in 18 chromosomes,and chloroplast members were located in chromosomes 6,17 and 19;phylo・genetic evolution showed that tobacco Hsp70 gene was distributed in 5 groups and 2 subgroups,and chloroplast members in V-A subgroup;gene structure showed that number of introns was 0-13,and number of introns was 7 in chloroplast;conserved motif revealed that Motif differed between groups,specific Motif 16,Motif 17 and Motif 20 were located in cytoplasmic members of group I,Motif and Motif 5 belonged to cytoplasm of group HI and endoplasmic reticulum of group IV,and Motif 15 and Motif 19 were present in V-A subgroup of chloroplast members;real-time fluorescence quantitative PCR analysis showed that NtCpHsp70 gene was not susceptible to high temperature induction.In summary,functional structure of tobacco Hsp70 is diverse,while expression of NtCpHsp70 gene is less affected by baking environment.

关 键 词：烟草 热激蛋白70 基因家族 生物信息学 叶绿体 

分 类 号：S572[农业科学—烟草工业] Q786[农业科学—作物学]