脐带间充质干细胞移植治疗心肌梗死大鼠心功能变化的Meta分析  

Changes of cardiac function in rats with myocardial infarction after umbilical cord-derived mesenchymal stem cell transplantation: a meta-analysis

作　　者：陈思宇 张涛[2] 殷文娟 蔡磊 李燕楠 颉丽英 左琳 Chen Siyu;Zhang Tao;Yin Wenjuan;Cai Lei;Li Yannan;Xie Liying;Zuo Lin(Key Laboratory of Cellular Physiology of Ministry of Education,Shanxi Provincial Key Laboratory,Department of Physiology,Shanxi Medical University,Taiyuan 030001,Shanxi Province,China;Department of Applied Psychology,Shanxi Medical University,Taiyuan 030001,Shanxi Province,China;School of Basic Medical Sciences,Shanxi Medical University,Taiyuan 030001,Shanxi Province,China)

机构地区：[1]细胞生理学教育部重点实验室,山西省重点实验室,山西医科大学生理学系,山西省太原市030001 [2]山西医科大学应用心理学专业,山西省太原市030001 [3]山西医科大学基础医学院,山西省太原市030001

出　　处：《中国组织工程研究》2020年第13期2126-2132,共7页Chinese Journal of Tissue Engineering Research

基　　金：山西省青年基金资助项目(201601D202106)，项目负责人：左琳;山西省回国留学人员科研基金资助项目(2014-036)，项目负责人：左琳;山西“1331工程”重点学科建设(1331KSC)(XK201708)，项目参与人：左琳~~

摘　　要：背景:研究显示,相对于其他来源的干细胞,脐带间充质干细胞这类多功能干细胞的免疫原性较低,将其应用到心肌梗死大鼠基础研究中具有显著疗效。目的:系统评价脐带间充质干细胞对心肌梗死大鼠心功能的影响。方法:计算机检索PubMed、Cochrane、Embase、CBM、中国知网、万方、维普、中国期刊数据库,检索时限从建库起至2019年6月,收集关于脐带间充质干细胞治疗大鼠心肌梗死的系列研究。由2位研究者按照纳入标准独立完成文献筛选、资料提取及方法学质量评价,采用Stata14.0进行Meta分析。结果与结论:最终9篇文献纳入研究,共计216只大鼠。Meta分析结果显示:①脐带间充质干细胞可显著升高心肌梗死大鼠的左室射血分数[95%CI(3.16,3.76),P<0.001];②脐带间充质干细胞对心肌梗死大鼠左室短轴缩短率有显著增加作用[95%CI(0.18,0.54),P<0.001];③脐带间充质干细胞对心肌梗死大鼠的左室舒张末期内径[95%CI(-1.90,-0.99),P=0.042]和收缩末期内径[95%CI(-6.56,-4.65),P<0.001]有明显缩短作用;④脐带间充质干细胞可显著改善心肌梗死大鼠的左室舒张末期容积[95%CI(-2.01,-1.11),P<0.001]和收缩末期容积[95%CI(-3.44,-2.17),P<0.001];⑤结果表明,脐带间充质干细胞移植治疗对心肌梗死大鼠的心功能有明显改善作用,且安全性较高。受纳入文献质量的限制,以上结论需更高质量、更大样本的随机对照实验加以验证。BACKGROUND:It has been shown that compared with stem cells from other sources,umbilical cord-derived mesenchymal stem cells have lower immunogenicity,and their application has significant effect in rats with myocardial infarction.OBJECTIVE:To systematically evaluate the effects of umbilical cord-derived mesenchymal stem cells on cardiac function of rats with myocardial infarction.METHODS:PubMed,Cochrane,Embase,CBM,CNKI,WanFang,VIP and CJD databases were retrieved for the literature concerning umbilical cord-derived mesenchymal stem cells for treating rats with myocardial infarction published before June 2019.Two researchers independently completed literature screening,data extraction and methodological quality evaluation according to the inclusion criteria.Meta-analysis was conducted on Stata 14.0.RESULTS AND CONCLUSION:A total of 9 articles were included,involving 216 rats.Meta-analysis showed that:(1)Umbilical cord-derived mesenchymal stem cells significantly increased the left ventricular ejection fraction after myocardial infarction in rats[95%confidence interval(CI)(3.16,3.76),P<0.001].(2)Umbilical cord-derived mesenchymal stem cells significantly increased the left ventricular short axis shortening rate after myocardial infarction in rats[95%CI(0.18,0.54),P<0.001].(3)Umbilical cord-derived mesenchymal stem cells significantly shortened the left ventricular end-diastolic internal diameter[95%CI(-1.90,-0.99),P=0.042]and left ventricular end-systolic internal diameter[95%CI(-6.56,-4.65),P<0.001].(4)Umbilical cord-derived mesenchymal stem cells significantly improved the left ventricular end-diastolic volume[95%CI(-2.01,-1.11),P<0.001]and left ventricular end-systolic volume[95%CI(-3.44,-2.17),P<0.001].In summary,umbilical cord-derived mesenchymal stem cell transplantation is effective and safe in the treatment of myocardial infarction in rats.Due to the limitation of the quality of the included literature,the above conclusions need to be validated by high-quality and large-sample randomized controlled trials.

关 键 词：脐带来源间充质干细胞 大鼠 心肌梗死 动物实验 心功能指数 META分析 

分 类 号：R459.9[医药卫生—治疗学] R318.11[医药卫生—临床医学]