中药石苦秦散及其有效成分对水禽源细菌的体外抑菌效果  

Antibacterial Activity in Vitro of Shikuqin Powder and Its Active Ingredients against Waterfowl-origin Bacteria

作　　者：张亚楠 徐景峨 蒲龄 彭珊 田青青 杨莉 王鑫 姜玲玲 余波 ZHANG Ya’nan;XU Jing’e;PU Ling;PENG Shan;TIAN Qingqing;YANG Li;WANG Xin;JIANG Lingling;YU Bo(Institute of Animal Husbandry and Veterinary Medicine,Guizhou Academy of Agricultural Sciences,Guiyang 550005,China;Guizhou Education University,Guiyang 550000,China)

机构地区：[1]贵州省农业科学院畜牧兽医研究所,贵州贵阳550005 [2]贵州师范学院,贵州贵阳550000

出　　处：《河南农业科学》2020年第1期128-136,共9页Journal of Henan Agricultural Sciences

基　　金：贵州省科技计划项目(黔科合支撑[2019]2287号);贵州省农业科技攻关项目(黔科合NY[2015]3009-2号);贵州省重大科技专项(黔科合重大专项字[2013]6014号);国家水禽产业技术体系贵阳综合试验站项目(CARS-42-53);贵州省农科院创新专项(黔农科院科技创新[2017]03号)

摘　　要：为研究中药复方制剂石苦秦散及其主要活性成分单体对水禽源多重耐药菌株的体外抑菌效果,采用K-B法对试验菌株进行耐药性检测,并采用微量肉汤二倍稀释法和琼脂平板培养计数法研究石苦秦散及其6种主要活性成分单体对鸭疫里氏杆菌、大肠杆菌、沙门氏菌、多杀性巴氏杆菌、金黄色葡萄球菌5种细菌的体外抑菌作用和杀菌作用。结果显示,90%的分离株为多重耐药菌株,肠杆菌属的大肠杆菌和沙门氏菌分离株耐药性最强,耐药种数均高于8种。石苦秦散对5种细菌的最小抑菌浓度(MIC)介于1.95~62.50 g/L,最小杀菌浓度(MBC)介于1.95~125.00 g/L;其中,对鸭疫里氏杆菌和多杀性巴氏杆菌的MIC较低,介于1.95~15.63 g/L。6种中药单体中,抑菌能力最强的为秦皮乙素,其次为苦参碱、没食子酸;6种中药单体杀菌效果存在较大差异,当质量浓度达到31.25 g/L时,没食子酸能杀死所有供试菌株,整体杀菌效果优于其他5种单体;6种中药单体对鸭疫里氏杆菌的MIC和MBC均小于其他菌株。由此可见,石苦秦散对供试的5种细菌均有抑菌作用和杀菌作用,其中对鸭疫里氏杆菌和多杀性巴氏杆菌的效果最好;6种中药单体对鸭疫里氏杆菌的抑菌效果和杀菌效果均优于其他菌种。In order to study the antibacterial activity in vitro of Shikuqin powder and its main active component monomers on waterfowl-origin multidrug-resistant strains,in this study,K-B method was used to detect the antibacterial agent resistance of the tested strains.Double dilution method of micro broth and agar plate culture counting method were used to investigate the antibacterial activity or bactericidal activity of Shikuqin powder and its main active component monomers on Riemerella anatipestifer,Escherichia coli,Salmonella,Pasteurella multocida and Staphylococcus aureus.The results showed that 90%of the isolates were multi-drug resistant strains.Escherichia coli and Salmonella isolates of Enterobacter were the most antibiotic resistant strains,and the number of antibiotic-resistant strains was higher than 8 kinds.The MICs of Shikuqin powder for five kinds of bacteria ranged from 1.95 g/L to 62.50 g/L,and MBCs ranged from 1.95 g/L to 125.00 g/L.The MICs of Shikuqin powder for Riemerella anatipestifer and Pasteurella multocida were lower,ranging from 1.95 g/L to 15.63 g/L.Among the six Chinese herbal monomers,esculetin was the strongest,followed by matrine and gallic acid.The bactericidal efficacy of the six Chinese herbal monomers varied greatly.When the concentration reached 31.25 g/L,gallic acid could kill all the tested strains,and the overall bactericidal efficacy was better than the other five monomers.The MIC and MBC of the six Chinese herbal monomers against Riemerella anatipestifer were lower than those of other strains.It can be seen that Shikuqin powder has antibacterial effect on these five kinds of bacteria,among which the bacteriostasis effect on Riemerella anatipestifer and Pasteurella multocida is the best.The bacteriostatic and bactericidal effects of six Chinese herbal monomers on Riemerella anatipestifer were superior to those of other strains.

关 键 词：石苦秦散复方制剂 鸭疫里氏杆菌 多杀性巴氏杆菌 MIC MBC 

分 类 号：S853.73[农业科学—临床兽医学]