基于正交试验和响应曲面法优化复方金钱草颗粒提取工艺  

Optimization of the compound herba lysimachiae extraction prosess based on orthogonal experiment and response surface method

作　　者：潘鹏超 陈啸飞 谌卫 郭志勇 柴逸峰[1] PAN Pengchao;CHEN Xiaofei;CHEN Wei;GUO Zhiyong;CHAI Yifeng(Department of Pharmaceutical Analysis,School of Pharmacy,Naval Medical University,Shanghai 200433,China;Department of Nephrology,Changhai Hospital,Naval Medical University,Shanghai 200433,China)

机构地区：[1]海军军医大学药学院药物分析教研室,上海200433 [2]海军军医大学长海医院肾内科,上海200433

出　　处：《药学实践杂志》2020年第1期27-34,共8页Journal of Pharmaceutical Practice

基　　金：国家自然科学基金(81573396,81973291,81573759)

摘　　要：目的采用正交试验和响应面分析法优化复方金钱草颗粒的提取工艺。方法以复方金钱草颗粒中多个代表性成分的峰面积之和为评价指标,应用正交试验及Box-Behnken响应面分析法对超声提取时间、超声温度以及乙醇比例3个提取条件进行3因素3水平回归分析,优化最佳提取条件。结果超声提取时间和乙醇比例是复方金钱草颗粒提取工艺的重要因素,超声温度对其提取效果作用不明显。复方金钱草颗粒的最佳提取条件为超声提取时间10 min,超声温度55℃,乙醇比例55%。结论结合正交试验以及Box-Behnken响应面分析法得到的最佳提取工艺参数,可用于复方金钱草颗粒代表性成分的提取以及后续的药学研究。Objective To optimize the extraction process of the compound herba lysimachiae by orthogonal experiment and response surface method.Methods The sum of the peak areas of multiple representative components in the compound herba lysimachiae was used as the evaluation index.The ultrasonic extraction time,ultrasonic temperature and ethanol ratio were analyzed to optimize the extraction process conditions by orthogonal experiment and Box-Behnken response surface analysis method.Results Ultrasonic extraction time and ethanol ratio are important factors in the extraction process of the compound herba lysimachiae.The effect of ultrasonic temperature in the extraction process is not significant.The optimal extraction condition for compound herba lysimachiaeare is 10 min ultrasonic extraction time at 55℃with 55%ethanol.Conclusion The optimal extraction process parameters obtained by combining orthogonal experiment and Box-Behnken response surface method can be used for the extraction of representative components from the compound herba lysimachiae and pharmaceutical research in the future.

关 键 词：复方金钱草颗粒 提取工艺 正交试验 响应面分析法 

分 类 号：R284.2[医药卫生—中药学]