紫外-可见分光光度法测定防暑清热饮中总黄酮和总多糖含量  

Content determination of total flavones and polysaccharides in Fangshu Qingre mixture by UV-Vis spectrophotometry

作　　者：李杰 杨育儒 王庆芬 郑绍忠 陈锦珊 LI Jie;YANG Yuru;WANG Qingfen;ZHENG Shaozhong;CHEN Jinshan(Department of Pharmacy,909th Hospital of Joint Service Support Force/Affiliated Southeast Hospital of Xiamen University,Zhangzhou 363000,China)

机构地区：[1]中国人民解放军联勤保障部队第九〇九医院/厦门大学附属东南医院制剂科福建

出　　处：《药学实践杂志》2020年第1期63-66,共4页Journal of Pharmaceutical Practice

基　　金：医院青年苗圃基金(17Y019)

摘　　要：目的建立紫外-可见分光光度法测定防暑清热饮中总黄酮和总多糖含量的方法。方法采用紫外-可见分光光度法,以芦丁、无水葡萄糖为对照品,在波长分别为508、487 nm处测定防暑清热饮中总黄酮和总多糖的含量。结果防暑清热饮中总黄酮含量在0.00~59.20μg/ml范围内,平均加样回收率为104.4%(n=6,RSD为1.11%);总多糖含量在10.92~109.20μg/ml,平均加样回收率为104.8%(n=6,RSD为1.88%)。结论采用紫外-可见分光光度法测定防暑清热饮中总黄酮和总多糖含量的方法操作简便、重复性好、准确可靠,可作为防暑清热饮中总黄酮和总多糖含量的测定方法。Objective To establish a method for content determination of total flavones and polysaccharides in Fangshu Qingre mixture by UV-Vis Spectrophotometry.Methods The contents of total flavones and polysaccharides in Fangshu Qingre mixture were determined by UV-Vis spectroscopy with rutin and anhydrous glucose as reference substance,and the wavelength was set at 508 nm and 487 nm.Results The contents were from 0.00 to 59.20μg/ml for total flavones and from 10.92 to 109.20μg/ml for total polysaccharides in Fangshu Qingre mixture.The recoveries of total flavones and total polysaccharides were 104.4%and 104.8%respectively.Conclusion The method of using ultraviolet spectroscopy was simple,reproducible,accurate and reliable,which could be preferably used as the method for content determination of total flavones and polysaccharides in Fangshu Qingre mixture.

关 键 词：紫外-可见分光光度法 防暑清热饮 总黄酮 总多糖 芦丁 无水葡萄糖 

分 类 号：R284.1[医药卫生—中药学]