烟草青枯病菌噬菌体分离及其生物学特性研究  

Isolation and Biological Characteristics of Tobacco Ralstonia solanacearum Bacteriophage

作　　者：胡蓉花 余成鹏 任敏华 李小勇 刘琼光[2,4] HU Ronghua;YU Chengpeng;REN Minhua;LI Xiaoyong;LIU Qiongguang(Ji'an Tobacco Company,Jiangxi Province,Ji'an 343000,China;College of Agriculture,South China Agricultural University,Guangzhou 510642,China;Xiajiang Tobacco Company,Jiangxi Province,Xiajiang 331400,China;Guangdong Provincial Key Laboratory of Microbial Signals and Disease Control,Guangzhou 510642,China.)

机构地区：[1]江西省吉安烟草公司,江西吉安343000 [2]华南农业大学农学院,广东广州510642 [3]江西省峡江烟草公司,江西峡江331400 [4]广东省微生物信号与作物病害防控重点实验室,广东广州510642

出　　处：《广东农业科学》2019年第11期78-84,共7页Guangdong Agricultural Sciences

基　　金：中国烟草总公司江西省公司科技项目(赣烟司[2016]81号)

摘　　要：【目的】烟草青枯病是我国南方烟区发生普遍而严重的细菌病害,目前尚未有理想的化学防治药剂,筛选青枯病菌噬菌体,为作物青枯病的防治提供噬菌体资源。【方法】从江西省吉安市烟田采集土壤样本,采用双层平板培养分离方法,分离青枯菌噬菌体,进一步比较物理和化学因素对噬菌体活性的影响。【结果】从吉安烟田土壤中分离获得6个青枯菌噬菌体,编号为PTb7-1、PTb1521、PTb1553、P1Tb1556、P2Tb1556和PTb574,其生物学特性存在差异。结果显示,多个噬菌体的最适生长温度约为35℃,致死温度为55~60℃,适宜酸碱度为pH 5~9,且对紫外光、乙醚和氯仿敏感。然而,噬菌体PTb7-1只适合于酸性环境条件下生长繁殖,噬菌体P2Tb1556对紫外光和乙醚不敏感,但对氯仿敏感。【结论】烟草土壤中具有丰富的青枯菌噬菌体资源,是筛选噬菌体的材料来源。6个青枯菌噬菌体生物学特性存在差异,噬菌体P2Tb1556对紫外光和乙醚不敏感,但对氯仿敏感。【Objective】Tobacco bacterial wilt a widespread and devastating bacterial disease in southern tobacco production area of China,and there is no effective chemical for the control of such disease.The screening of phage will provide phage resources for the prevention and control of tobacco bacterial wilt in the future.【Method】Soil samples were collected from the tobacco field of Ji'an City,Jiangxi Province,and the bacterial wilt phages were isolated by double-layer plate culture and isolation method and the effects of physical and chemical factors on the activity of phage were further compared.【Result】Six Ralstonia solanacearum bacteriophages were isolated from the soil of tobacco field in Ji'an City,and numbered as PTb7-1,PTb1521,PTb1553,P1Tb1556,P2Tb1556 and PTb574,among which there were differences in biological characteristics.The results of biological characteristics research showed that the optimum growth temperature for several phages was about 35℃,the lethal temperature was 55-60℃and the optimum pH was 5-9,and those phages were sensitive to ultraviolet,ether and chloroform.However,phage PTb7-1 was only suitable to grow and propagate under acidic environment.Phage P2Tb1556 was not sensitive to ultraviolet and ether,but highly sensitive to chloroform.【Conclusion】There are abundant phage resources in tobacco soil,which is a good source for phage screening.The biological characteristics of the six phages is different.P2Tb1556 phage is not sensitive to ultraviolet light and ether,but sensitive to chloroform.

关 键 词：茄科劳尔氏细菌 烟草 噬菌体 温度 紫外光 氯仿 乙醚 

分 类 号：S432.42[农业科学—植物病理学]