三氯生对雌性斑马鱼肝胰脏凋亡相关基因表达的影响  

Effect of Triclosan on Expression of Apoptosis-related Genes in Female Zebrafish Hepatopancreas

作　　者：刘旭昊[1] 祖恩普[1] 王凡[1] LIU Xu-hao;ZU En-pu;WANG Fan(Life Science Department,Luoyang Normal University,Luoyang 471022,China)

机构地区：[1]洛阳师范学院

出　　处：《广东海洋大学学报》2020年第1期15-18,共4页Journal of Guangdong Ocean University

基　　金：国家自然科学基金(31971524);NSFC-河南人才培养联合基金(U1504303)

摘　　要：【目的】研究三氯生(TCS)对雌性硬骨鱼类肝胰脏损伤的相关分子机制。【方法】采用半静态水体接触染毒法,将雌性斑马鱼(Danio rerio)暴露于0、0.017、0.034、0.068 mg/L的TCS溶液42 d,采用普通PCR和实时荧光定量PCR技术测定雌性斑马鱼肝胰脏凋亡相关基因(Bcl-2、p53、MDM2、Bax)的表达情况。【结果】0.017~0.068 mg/L组的雌性斑马鱼肝胰脏Bcl-2、MDM2和Bax基因的表达极显著下调(P<0.01),p53基因的表达极显著上调(P<0.01)。【结论】0.017~0.068 mg/L的TCS显著影响雌性斑马鱼肝胰脏凋亡相关基因的表达,促进雌性斑马鱼肝胰脏细胞凋亡的发生。【Objective】To study the molecular mechanism of triclosan(TCS)on hepatopancreas injury in the female bony fish.【Method】Zebrafish(Danio rerio)were exposed to TCS solution of 0,0.017,0.034,and 0.068 mg/L respectively by semi-static water exposure for 42 days,and the expressions of apoptosis-related gene(Bcl-2,p53,MDM2,and Bax)in the hepatopancreas were detected by PCR and real-time quantitative PCR.【Results】Compared with the control group,the expressions of Bcl-2,MDM2 and Bax gene in the TCS treated groups were significantly down-regulated(P<0.01),and P53 gene was significantly up-regulated in the TCS treated group(P<0.01).【Conclusion】TCS has a significant effect on the expression of apoptosis-related genes in the hepatopancreas of zebrafish and may accelerate apoptosis in the hepatopancreas of female zebrafish.

关 键 词：三氯生 斑马鱼 雌性斑马鱼 肝胰脏损伤 凋亡基因 实时荧光定量PCR 

分 类 号：X503.225[环境科学与工程—环境工程] Q786[生物学—分子生物学]