我国蔗区甘蔗宿根矮化病发生情况的分子检测  

Molecular Detection of Sugarcane Ratoon Stunting Disease in Sugarcane Producing Areas of China

作　　者：沈林波[1] 吴楠楠 冯小艳[1] 王文治[1] 熊国如[1] 赵婷婷[1] 张树珍[1] SHEN Linbo;WU Nannan;FENG Xiaoyan;WANG Wenzhi;QIONG Guoru;ZHAO Tingting;ZHANG Shuzhen(Sugarcane Research Center,Chinese Academy of Tropical Agricultural Sciences/Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences/Ministry of Agriculture and Rural Affairs Key Laboratory of Tropical Crop Biology and Genetic Resources,Haikou,Hainan 571101,China;College of Life Sciences,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]中国热带农业科学院甘蔗研究中心/热带生物技术研究所/农业部热带作物生物学与遗传资源利用重点实验室,海口571101 [2]南京农业大学生命科学学院,南京210095

出　　处：《热带生物学报》2019年第4期314-318,共5页Journal of Tropical Biology

基　　金：国家自然科学基金项目(31771865);海南省科协青年科技英才创新计划(HAST201626);国家糖料产业技术体系“甘蔗病毒病防控岗位科学家”经费(CARS-170301)

摘　　要：为明确我国甘蔗产区(简称蔗区)甘蔗宿根矮化病(Sugarcane Ratoon Stunting Disease,RSD)的发生情况,从我国甘蔗主产区的12个蔗区收集甘蔗叶片样品598份,采用特异性引物通过聚合酶链反应(polymerase chain reaction,PCR)方法进行宿根矮化病的分子检测。结果表明,598份甘蔗样品中有99份能检测到RSD,平均检出率为16.56%。12个蔗区的RSD检出率各不相同,临沧蔗区的RSD检出率最高,为27.78%;南宁蔗区的RSD检出率最低,为4.88%。15个主要甘蔗栽培品种均能检测到RSD,阳性检出率为15.38%~44.44%,其中新台糖22号RSD发病最严重,阳性检出率为44.44%,海蔗22号RSD发病最轻,阳性检出率为15.38%。可见,宿根矮化病在我国甘蔗栽培品种中普遍发生。In order to clarify the occurrence of sugarcane ratoon stunting disease(RSD) in sugarcane plantations in China, 598 sugarcane leaf samples were collected from 12 sugarcane plantations in the main sugarcane producing areas like Guangxi, Yunnan, Guangdong and Hainan in China, and the pathogen of RSD was identified by using specific primers through polymerase chain reaction(PCR). The results showed that 99 out of 598 sugarcane samples were detected to contain bacteria of RSD, with an average detection rate of 16.56%. RSD detection rates varied in 12 sugarcane plantations, the highest in Lincang sugarcane plantations(27.78%) and the lowest in Nanning sugarcane plantations(4.88%). RSD was detected in 15 main sugarcane cultivars, with positive detection rates ranging from 15.38% to 44.44%. Among the sugarcane cultivars, sugarcane cultivar ROC 22 was most seriously infected with RSD, and its RSD positive detection rate was 44.44%, while sugarcane cultivar Haitang 22 was less infected with RSD and its RSD positive detection rate was 15.38%. It can be seen that sugarcane ratoon stunting disease is common in sugarcane cultivars in China.

关 键 词：甘蔗 宿根矮化病 分子检测 检出率 

分 类 号：S566.1[农业科学—作物学]