濒危兰科植物DNA条形码鉴定体系的建立与优化  

Establishment and Optimization of DNA Barcoding in Endangered Orchid Plants

作　　者：潘英文 张凌 王安石 李佳潼 谢添伟 陈施明 刘福秀 林明光 PAN Yingwen;ZHANG Ling;WANG Anshi;LI Jiatong;XIE Tianwei;CHEN Shiming;LIU Fuxiu;LIN Mingguang(Post-Entry Quarantine Station for Tropical Plants,Haikou Customs,Haikou,Hainan 570311,China)

机构地区：[1]海口海关热带植物隔离检疫中心

出　　处：《热带生物学报》2019年第4期319-323,共5页Journal of Tropical Biology

基　　金：海南省自然科学基金项目(317293);国家质量监督检验检疫总局科技计划项目(2016IK095)

摘　　要：通过设计濒危兰科植物DNA条形码ITS,matK和psbA-trnH序列的通用引物并对其PCR反应体系进行优化,对16种有代表性的濒危兰科植物进行PCR扩增和测序,比较各序列的扩增和测序效率。结果表明:ITS,matK和psbA-trnH序列引物对濒危兰科植物的扩增和测序成功率均较高,PCR反应的最佳退火温度分别为ITS 54℃,matK和psbA-trnH 50℃。在上述反应体系和条件下获得的目的条带清晰、明亮,可作为鉴别濒危兰科植物物种的DNA条形码组合。Universal primers were designed for ITS, matK and psbA-trnH sequences of endangered orchids for DNA barcoding and their PCR reaction system optimized. Sixteen species of endangered orchids were amplified by using PCR and sequenced, and their PCR amplification and sequencing efficiencies were compared. The results showed that the primers for the ITS, matK and psbA-trnH sequences had higher amplification success rate and sequencing success rate for species of the endangered orchid plants. The optimum annealing temperatures for the PCR reaction were 54 ℃ for ITS and 50 ℃ for matK and psbA-trnH. The targeted bands obtained under the above reaction systems and conditions were clear and bright, and the ITS, matK and psbA-trnH sequences can be used as a combination of DNA barcodes to identify the species of endangered orchid plants.

关 键 词：濒危兰科植物 DNA条形码 鉴定 

分 类 号：S682.31[农业科学—观赏园艺]