大尖囊蝴蝶兰内生真菌和细菌的分离与鉴定  

Isolation and Identification of Endophytic Fungi and Bacteria from Phalaenopsis deliciosa

作　　者：陈耀丽 俞龙春 钱悦 宋希强[1] 张哲[1,2] CHEN Yaoli;YU Longchun;QIAN Yue;SONG Xiqiang;ZHANG Zhe(Ministry of Education Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants,College of Forestry,Hainan University,Haikou,Hainan 570228,China;Hainan Yaode Agricultural Science and Technology Co.LTD,Haikou,Hainan 570228,China)

机构地区：[1]海南大学林学院/热带特色林木花卉遗传与种质创新教育部重点实验室,海口570228 [2]海南耀德农业科技有限公司,海口570228

出　　处：《热带生物学报》2019年第4期372-379,共8页Journal of Tropical Biology

基　　金：海南省自然科学基金创新团队项目(2018CXTD331)

摘　　要：以大尖囊蝴蝶兰(Phalaenopsis deliciosa)为材料,采用常规无菌培养技术从其根部分离内生真菌和细菌并进行分子鉴定,旨在了解大尖囊蝴蝶兰内生真菌和细菌的特点及多样性。实验结果获得代表性内生真菌菌株38株,鉴定归为5个OTUs,隶属于1门2纲4目4科5属,优势属为球二孢属(Lasiodiplodia)和曲霉属(Aspergillus);获得代表性内生细菌菌株54株,鉴定归为17个OTUs,隶属于3门5纲6目9科12属,优势属为芽孢杆菌属(Bacillus)和肠杆菌属(Enterobacter)。研究结果可为建立大尖囊蝴蝶兰的共生萌发和菌根化育苗提供基础数据,也为内生微生物与兰科植物的关系研究提供基础资料。Endophytic fungi and bacteria are critical throughout the life cycle of Orchidaceae plants. Phalaenopsis deliciosa(Orchidaceae) was used as test material to isolate the endophytic fungi and bacteria from its fresh roots in routine sterile culture, and the isolates were then identified by using molecular markers to reveal their characteristics and diversity. The results showed that a total of 38 strains of endophytic fungi were isolated from the roots of P. deliciosa and identified as 5 OTUs belonging to 1 phylum, 2 classes, 4 orders, 4 families and 5 genera, and that the dominant genera were Lasiodiplodia and Aspergillus, while a total of 54 strins of endophytic bacteria were isolated and 17 OTUs were identified to belong to 3 phyla, 5 classes, 6 orders, 9 families, 12 genera, and that the dominant genera were Bacillus and Enterobacter. The results will provide reference for symbiotic germination and raising of mycorrhiza-based seedlings of P. deliciosa and for further study of the relationship between endophytic microorganisms and orchids.

关 键 词：蝴蝶兰属 内生真菌 内生细菌 分子鉴定 

分 类 号：S682.31[农业科学—观赏园艺]